{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T05:08:59Z","timestamp":1775624939165,"version":"3.50.1"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1985,5,1]],"date-time":"1985-05-01T00:00:00Z","timestamp":483753600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1985,5,1]]},"abstract":"<jats:p> The actions of a series of 15 Ca<jats:sup>2+<\/jats:sup> channel antagonists including D-6(X), nifedipine, and diltiazem were examined against K<jats:sup>+<\/jats:sup> depolarization and muscarinic receptor induced responses in guinea pig bladder smooth muscle. Responses of bladder are very dependent upon extracellular Ca<jats:sup>2+<\/jats:sup> and sensitive to the Ca<jats:sup>2+<\/jats:sup> channel antagonists, the tonic component more than the phasic component of response. Regardless of stimulant, K<jats:sup>+<\/jats:sup> or methylfurmethide (MF), or component of response, the same rank order of antagonist activities is expressed, suggestive of a single structure\u2013activity relationship and the existence of a single category of binding site which may, however, exist in several affinity states. High affinity binding of [<jats:sup>3<\/jats:sup>H]nitrendipine (K<jats:sub>D<\/jats:sub>\u2002=\u20021.1\u2002\u00d7\u200210<jats:sup>\u221210<\/jats:sup>\u2002M) occurs in bladder membranes, and similar high affinity binding was found in microsomal preparations from other smooth muscles including guinea pig and rat lung, rat vas deferens, uterus, and stomach. [<jats:sup>3<\/jats:sup>H]nitrendipine binding in the bladder was sensitive to displacement by other 1,4-dihydropyridines, paralleling their pharmacologic activities and showing excellent agreement with binding data previously obtained for guinea pig ileal smooth muscle. Comparison of pharmacologic data for inhibition of K<jats:sup>+<\/jats:sup>- and MF-induced responses by a common series of Ca<jats:sup>2+<\/jats:sup> channel antagonists in bladder and ileum revealed excellent correlations. Neither pharmacologic nor binding studies suggest significant differences in Ca<jats:sup>2+<\/jats:sup> channel antagonist properties in smooth muscle from bladder and intestine. <\/jats:p>","DOI":"10.1139\/y85-079","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:53:25Z","timestamp":1303635205000},"page":"453-462","source":"Crossref","is-referenced-by-count":25,"title":["Ca<sup>2+<\/sup> channel antagonist actions in bladder smooth muscle: comparative pharmacologic and [<sup>3<\/sup>H]nitrendipine binding studies"],"prefix":"10.1139","volume":"63","author":[{"given":"F. B.","family":"Yousif","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. T.","family":"Bolger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ruzycky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Triggle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y85-079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T16:21:55Z","timestamp":1578241315000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y85-079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1985,5,1]]}},"alternative-id":["10.1139\/y85-079"],"URL":"https:\/\/doi.org\/10.1139\/y85-079","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,5,1]]}}}