{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:06:27Z","timestamp":1762067187911},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1986,5,1]],"date-time":"1986-05-01T00:00:00Z","timestamp":515289600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1986,5,1]]},"abstract":"<jats:p> These studies were designed to examine the changes in the characteristics of prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) binding to porcine oxyntic mucosa in the response to oral ingestion of salicylates. Either acetylsalicylic acid (ASA) or salicylic acid (SA) was administered to conscious pigs (100\u2002mg\/kg in 30\u2002mL of an equimolar concentration of NaHCO<jats:sub>3<\/jats:sub>) once a day for 1, 3, 10, or 20 days. In control experiments a similar volume of 0.3\u2002M NaHCO<jats:sub>3<\/jats:sub> was administered for similar durations. Mucosal ulceration and the characteristics of the binding of [<jats:sup>3<\/jats:sup>H]PGE<jats:sub>2<\/jats:sub> to a 30\u2002000\u2002\u00d7\u2002g membrane preparation of oxyntic mucosa were examined. Generation of mucosal PGE<jats:sub>2<\/jats:sub> was measured by radioimmunoassay. ASA treatment resulted in an increase in the number and severity of mucosal ulcers and a decrease in PGE<jats:sub>2<\/jats:sub> levels within the first treatment day. By day 20 the degree of ulceration had decreased in spite of a persistent reduction of mucosal PGE<jats:sub>2<\/jats:sub> generation. A variable degree of ulceration was observed in SA-treated animals. In control animals only a single class of binding sites for [<jats:sup>3<\/jats:sup>H]PGE<jats:sub>2<\/jats:sub> was evident. After 3 days of ASA treatment a second class of binding sites with a high affinity dissociation constant appeared. There was a decrease in the high affinity binding of [<jats:sup>3<\/jats:sup>H]PGE<jats:sub>2<\/jats:sub> after 20 days of ASA ingestion. Low affinity binding was not altered. ASA treatment resulted in a significant increase in specific binding capacities for both families of binding sites. SA treatment did not consistently alter PGE<jats:sub>2<\/jats:sub> binding characteristics from control at any time period studied. These data suggest that SA treatment results in a small degree of mucosal damage in the absence of a significant reduction in tissue generation of PGE<jats:sub>2<\/jats:sub> or changes in PGE<jats:sub>2<\/jats:sub> binding. Damage in response to ASA ingestion was associated with a reduction in both endogenous synthesis of PGE<jats:sub>2<\/jats:sub> and an increase in the concentration of both low and high affinity binding sites for PGE<jats:sub>2<\/jats:sub>. The reduction in mucosal ulceration on day 20 in spite of depressed endogenous PGE<jats:sub>2<\/jats:sub> coincides with an increase in PGE<jats:sub>2<\/jats:sub> binding. <\/jats:p>","DOI":"10.1139\/y86-085","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:54:18Z","timestamp":1303635258000},"page":"515-520","source":"Crossref","is-referenced-by-count":3,"title":["Prostaglandin E<sub>2<\/sub> binding sites in porcine oxyntic mucosa: effects of salicylates"],"prefix":"10.1139","volume":"64","author":[{"given":"B. L.","family":"Tepperman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. D.","family":"Soper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y86-085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:18:43Z","timestamp":1578237523000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y86-085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1986,5,1]]}},"alternative-id":["10.1139\/y86-085"],"URL":"https:\/\/doi.org\/10.1139\/y86-085","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,5,1]]}}}