{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T14:29:35Z","timestamp":1764858575366},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1987,5,1]],"date-time":"1987-05-01T00:00:00Z","timestamp":546825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1987,5,1]]},"abstract":"<jats:p> Both surface pH (pH<jats:sub>s<\/jats:sub>) and intracellular pH (pH<jats:sub>i<\/jats:sub>) were measured using single- and double-barreled pH-sensitive microelectrodes in isolated sheep cardiac Purkinje strands, rabbit and cat papillary muscle, and mouse and rat soleus muscle. Superfusion of the preparations with a relatively low buffered solution (containing 5\u2002mM HEPES buffered to control pH) causes surface acidosis that correlates with efflux of metabolically produced acids in the unstirred layer of fluid surrounding the tissue. Acidification of the surface layer induces a slower acid change of pH<jats:sub>i<\/jats:sub> and depresses the rate of proton extrusion following an imposed intracellular acid load. In cardiac preparations, the lowering of pH<jats:sub>i<\/jats:sub> correlates with depression of twitch tension. Transient changes of pH<jats:sub>s<\/jats:sub> and pH<jats:sub>i<\/jats:sub> are seen when a weak acid or base is suddenly added to, or removed from the superfusion solution. Indirect evidence of the presence of carbonic anhydrase in the extracellular surface layer is obtained from analysis of transient pH<jats:sub>s<\/jats:sub> changes in presence and absence of acetazolamide. <\/jats:p>","DOI":"10.1139\/y87-154","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:54:53Z","timestamp":1303635293000},"page":"970-977","source":"Crossref","is-referenced-by-count":35,"title":["Surface pH and the control of intracellular pH in cardiac and skeletal muscle"],"prefix":"10.1139","volume":"65","author":[{"given":"A. de","family":"Hemptinne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Marrannes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Vanheel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y87-154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:18:11Z","timestamp":1578237491000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y87-154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1987,5,1]]}},"alternative-id":["10.1139\/y87-154"],"URL":"https:\/\/doi.org\/10.1139\/y87-154","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,5,1]]}}}