{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T03:04:13Z","timestamp":1648868653262},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[1987,10,1]],"date-time":"1987-10-01T00:00:00Z","timestamp":560044800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1987,10,1]]},"abstract":"<jats:p> The effect of BFE-55, a derivative of befunolol (a \u03b2-adrenergic partial agonist) on specific [<jats:sup>3<\/jats:sup>H]befunolol binding to a microsomal fraction from the guinea pig taenia caecum was tested. A Scatchard plot of specific [<jats:sup>3<\/jats:sup>H]befunolol binding in the absence of BFE-55 was concave, suggesting an existence of high and low affinity sites in \u03b2-adrenoceptors. In contrast, the Scatchard plot of data in the presence of BFE-55 (3\u2002\u00d7\u200210<jats:sup>\u22127<\/jats:sup>\u2002M) was straight. In the presence of BFE-55, the high affinity sites disappeared and the low affinity site was unaffected. In the absence and presence of BFE-55 there was no difference between the pK<jats:sub>D<\/jats:sub> values or B<jats:sub>max<\/jats:sub> values at the low affinity site. These findings indicate that BFE-55 interacts with only the high affinity sites in \u03b2-adrenoceptors. <\/jats:p>","DOI":"10.1139\/y87-338","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:55:33Z","timestamp":1303635333000},"page":"2150-2153","source":"Crossref","is-referenced-by-count":2,"title":["A derivative of befunolol, BFE-55, interacts with only the high affinity sites in \u03b2-adrenoceptors"],"prefix":"10.1139","volume":"65","author":[{"given":"Katsuo","family":"Koike","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Nakagoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issei","family":"Takayanagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y87-338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:17:29Z","timestamp":1578237449000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y87-338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1987,10,1]]}},"alternative-id":["10.1139\/y87-338"],"URL":"https:\/\/doi.org\/10.1139\/y87-338","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,10,1]]}}}