{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T17:24:26Z","timestamp":1697909066093},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1988,8,1]],"date-time":"1988-08-01T00:00:00Z","timestamp":586396800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1988,8,1]]},"abstract":"<jats:p> Previous studies provided strong evidence that propyl-methylenedioxyindene (pr-MDI) interfered with calcium at an intracellular site. To further characterize the mechanism of action of pr-MDI, its pharmacological actions on chemically skinned vascular smooth muscle were examined. Rat caudal artery strips were chemically skinned with saponin (0.15\u2002mg\/mL for 1\u2002h). The efficiency of the skinning was evidenced by a loss of contractile response to 74\u2002mM K<jats:sup>+<\/jats:sup>. The intactness of the regulatory and contractile proteins was ascertained by the ability of the skinned tissue to contract in response to Ca<jats:sup>2+<\/jats:sup> (free Ca<jats:sup>2+<\/jats:sup> concentration of 10<jats:sup>\u22124<\/jats:sup> or 10<jats:sup>\u22126<\/jats:sup>\u2002M). Caffeine (25\u2002mM) induced contraction was used as an index of the functional integrity of the sarcoplasmic reticulum in the skinned preparations. Contraction of the skinned artery with a free Ca<jats:sup>2+<\/jats:sup> concentration of 10<jats:sup>\u22126<\/jats:sup>\u2002M was significantly obtunded by 1\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M trifluoperazine (a calmodulin antagonist) but not by 1\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M pr-MDI. Contraction of the skinned artery evoked by 25\u2002mM caffeine in the absence of extracellular calcium was significantly obtunded by 1\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M pr-MDI but not by 1\u2002\u00d7\u200210<jats:sup>\u22126<\/jats:sup>\u2002M nifedipine (a calcium channel blocker). The results indicate that pr-MDI acts intracellularly to block calcium mobilization from the sarcoplasmic reticulum without directly interfering with the regulatory and contractile proteins. <\/jats:p>","DOI":"10.1139\/y88-170","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:56:10Z","timestamp":1303620970000},"page":"1041-1047","source":"Crossref","is-referenced-by-count":6,"title":["Pharmacological actions of the calcium antagonist propyl-methylenedioxyindene in skinned vascular smooth muscle"],"prefix":"10.1139","volume":"66","author":[{"given":"Wai-Shiu","family":"Wong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf G.","family":"Rahwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y88-170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T10:16:31Z","timestamp":1578219391000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y88-170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1988,8,1]]}},"alternative-id":["10.1139\/y88-170"],"URL":"https:\/\/doi.org\/10.1139\/y88-170","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,8,1]]}}}