{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T05:51:42Z","timestamp":1721800302025},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1989,5,1]],"date-time":"1989-05-01T00:00:00Z","timestamp":609984000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1989,5,1]]},"abstract":"<jats:p> The objective of this study was to investigate under controlled conditions the effects of fentanyl on the rat locus coeruleus catechol oxidation current. Using differential normal pulse voltammetry combined with electrochemically treated carbon fiber electrodes to measure the catechol oxidation current, catecholamine metabolism can be reliably monitored. Male Sprague\u2013Dawley rats weighing 500\u2013600\u2002g had carbon fiber electrodes implanted into the locus coeruleus under halothane \u2013 O<jats:sub>2<\/jats:sub> \u2013 air anesthesia with controlled ventilation and muscle relaxation. Experiments consisted of four groups of rats given the following treatments: (A) saline (n\u2002=\u20026); (B) fentanyl, 10\u2002\u03bcg\u2219kg<jats:sup>\u22121<\/jats:sup>\u2002i.v. (n\u2002=\u20026); (C) naloxone, 800\u2002\u03bcg\u2219kg<jats:sup>\u22121<\/jats:sup>\u2002i.v. followed 2\u2002min later by fentanyl, 10\u2002\u03bcg\u2219kg<jats:sup>\u22121<\/jats:sup> (n\u2002=\u20025); (D) clonidine, 200\u2002\u03bcg\u2219kg<jats:sup>\u22121<\/jats:sup>\u2002i.p. (n\u2002=\u20026). There was no significant change in the catechol oxidation current following saline. Fentanyl produced a significant (ANOVA, p\u2002&lt;\u20020.05) decrease in the catechol oxidation current (maximum 32\u2002min postinjection was 75.8\u2002\u00b1\u20024.6% of baseline). This decrease was prevented by a prior injection of naloxone. Clonidine produced a significant decrease in catechol oxidation current (maximum 40\u2002min postinjection was 54.1\u2002\u00b1\u20027.0% of baseline). Systolic blood pressure was significantly decreased following clonidine and there were no significant changes in arterial blood gases throughout the experiments. The \u03b1<jats:sub>2<\/jats:sub>-adrenergic agonist clonidine and the opioid fentanyl produced a decrease in locus coeruleus catechol oxidation current measured by in vivo voltammetry, which monitors catecholamine turnover.Key words: catecholamine, clonidine, fentanyl, opiates, locus coeruleus, in vivo voltammetry. <\/jats:p>","DOI":"10.1139\/y89-085","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:56:31Z","timestamp":1303635391000},"page":"532-536","source":"Crossref","is-referenced-by-count":10,"title":["Fentanyl decreases catecholamine metabolism measured by in vivo voltammetry in the rat locus coeruleus"],"prefix":"10.1139","volume":"67","author":[{"given":"Brian","family":"Milne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Quintin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Yves","family":"Gillon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Fran\u00e7ois","family":"Pujol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y89-085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:14:23Z","timestamp":1578237263000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y89-085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,5,1]]}},"alternative-id":["10.1139\/y89-085"],"URL":"https:\/\/doi.org\/10.1139\/y89-085","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,5,1]]}}}