{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T20:49:30Z","timestamp":1726087770308},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1990,8,1]],"date-time":"1990-08-01T00:00:00Z","timestamp":649468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1990,8,1]]},"abstract":"<jats:p> Possible interactions between \u03b1-latrotoxin, an activator of synaptosomal calcium uptake, and \u03c9-conotoxin GVIA, an inhibitor of voltage-sensitive calcium channels of the N-type, were investigated in rat and chicken synaptosomal preparations. While \u03c9-conotoxin GVIA potently and effectively inhibited calcium uptake induced by elevated potassium in chick synaptosomes, little or no effect of \u03c9-conotoxin GVIA was observed either in potassium-treated rat synaptosomes or in \u03b1-latrotoxin-exposed synaptosomes of either species. In contrast to the lack of effect of \u03c9-conotoxin on stimulated calcium uptake in rat synaptosomes, cadmium effectively inhibited calcium uptake induced by either potassium or \u03b1-latrotoxin. Synaptosomal calcium transport induced by \u03b1-latrotoxin can be bidirectional, since \u03b1-latrotoxin also induced efflux of preaccumulated calcium. Competition experiments revealed that binding of <jats:sup>125<\/jats:sup>I-labelled \u03c9-conotoxin and <jats:sup>125<\/jats:sup>I-labelled \u03b1-latrotoxin was similar in either chicken or rat synaptosomes. Neither \u03b1-latrotoxin nor \u03c9-conotoxin competed with the binding of the other ligand in either species. The results reported here show that (1) elevated potassium evokes calcium uptake principally through N-channels in avian but not in rat synaptosomes; (2) \u03b1-latrotoxin-activated calcium fluxes are \u03c9-conotoxin insensitive but cadmium sensitive; (3) the molecular acceptors for the two ligands are likely to be unrelated synaptic membrane constituents.Key words: synaptosom\u03b1es, calcium, \u03b1-latrotoxin, \u03c9-conotoxin. <\/jats:p>","DOI":"10.1139\/y90-158","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:57:10Z","timestamp":1303621030000},"page":"1049-1054","source":"Crossref","is-referenced-by-count":14,"title":["Interactions between the presynaptically active neurotoxins \u03b1-latrotoxin and \u03c9-conotoxin GVIA: studies on calcium fluxes and binding parameters in rat and chicken synaptosomes"],"prefix":"10.1139","volume":"68","author":[{"given":"H. W.","family":"Scheer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y90-158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T10:22:57Z","timestamp":1578219777000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y90-158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1990,8,1]]}},"alternative-id":["10.1139\/y90-158"],"URL":"https:\/\/doi.org\/10.1139\/y90-158","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8,1]]}}}