{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:03:16Z","timestamp":1778274196993,"version":"3.51.4"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1990,8,1]],"date-time":"1990-08-01T00:00:00Z","timestamp":649468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1990,8,1]]},"abstract":"<jats:p> Changes in systemic acid \u2013 base balance are known to influence acidification in the collecting duct. The H<jats:sup>+<\/jats:sup> secretion in the collecting duct has been shown to be an electrogenic process and it has been suggested that an H-ATPase sensitive to inhibition by N-ethylmaleimide (NEM) is responsible for H<jats:sup>+<\/jats:sup> secretion. This study was designed to determine the effect of metabolic alkalosis on NEM-sensitive ATPase activity in the microdissected segments of the distal nephron. Metabolic alkalosis was produced by giving NaHCO<jats:sub>3<\/jats:sub> to normal rats for 7 days. The plasma total CO<jats:sub>2<\/jats:sub> concentration in the experimental group was 31.5\u2002\u00b1\u20021.8\u2002mM compared with 23.4\u2002\u00b1\u20021.0\u2002mM in the control group. NEM-sensitive ATPase activity was significantly lower in the cortical collecting duct and in the outer and inner medullary collecting ducts of alkali-loaded rats than those of control rats. There was no significant difference in the enzyme activity between the two groups of animals in the other nephron segments examined. Our results suggest that NEM-sensitive H-APTase activity in all three segments of the collecting duct is modulated by the acid \u2013 base status of the animal.Key words: collecting duct, H-ATPase, electrogenic H-pump, metabolic alkalosis, rat kidney. <\/jats:p>","DOI":"10.1139\/y90-167","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:57:10Z","timestamp":1303635430000},"page":"1119-1123","source":"Crossref","is-referenced-by-count":9,"title":["Decrease in <i>N<\/i>-ethylmaleimide-sensitive ATPase activity in collecting duct by metabolic alkalosis"],"prefix":"10.1139","volume":"68","author":[{"given":"Lal C.","family":"Garg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelam","family":"Narang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y90-167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:23:03Z","timestamp":1578237783000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y90-167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1990,8,1]]}},"alternative-id":["10.1139\/y90-167"],"URL":"https:\/\/doi.org\/10.1139\/y90-167","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8,1]]}}}