{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T08:49:11Z","timestamp":1725785351498},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[1991,2,1]],"date-time":"1991-02-01T00:00:00Z","timestamp":665366400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:p> We examined whether drugs that block calcium, prostaglandins, free radicals, and endorphin release could modify cerebral blood flow or nerve tissue pathology following a focal cerebrovascular lesion. Cats were randomly divided into six groups and were subjected to standard middle cerebral artery occlusion (MCAO) performed using a transorbital approach. One hour after MCAO, cats received the following compounds intravenously: (i) saline (CS), 1.5\u2002mL\/kg or polyethylene glycol, 300\u2002\u03bcg (CP); (ii) naloxone (NX), 2\u2002mg\/kg; (iii) nimodipine (NM), 1\u2002\u03bcg\u2219kg<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup>\u2002\u00d7\u200260\u2002min; (iv) dimethyl sulfoxide (DS), 0.9\u2002g\/kg in a 40% solution; (v) prostacyclin (PGI<jats:sub>2<\/jats:sub>), 200\u2002ng\u2219kg<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup> for 60\u2002min; or (vi) DS\u2013PGI<jats:sub>2<\/jats:sub> combined. At 1-h intervals, local CBF was recorded from the cortical tissue proximal and distal to the MCAO site using the hydrogen clearance method. Five hours after MCAO, cortical tissue was removed for catecholamine histofluorescence or perfused for tyrosine hydroxylase immunoreactive axon examination. Treatment with NX, NM, CP, or CS had no effect on either CBF or cortical tissue neurotransmitter morphology. PGI<jats:sub>2<\/jats:sub> showed a transiently modest but significant increase of CBF, while DS provided moderate protection of catecholaminergic fibers and increased CBF by 27% after MCAO. The combination of DS\u2013PGI<jats:sub>2<\/jats:sub> resulted in significant cytoprotection of cortical catecholaminergic fibers and generated a sustained CBF increase of 68% of control values. These findings suggest that combining DS with PGI<jats:sub>2<\/jats:sub> can yield a synergic effect with respect to cortical neurotransmitter and CBF protection after MCAO.Key words: dimethyl sulfoxide, prostacyclin, cerebral ischemia, nimodipine, naloxone. <\/jats:p>","DOI":"10.1139\/y91-028","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:57:55Z","timestamp":1303635475000},"page":"191-198","source":"Crossref","is-referenced-by-count":12,"title":["Synergic activity of combined prostacyclin: dimethyl sulfoxide in experimental brain ischemia"],"prefix":"10.1139","volume":"69","author":[{"given":"J. C. de la","family":"Torre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y91-028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:23:25Z","timestamp":1578237805000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y91-028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,2,1]]}},"alternative-id":["10.1139\/y91-028"],"URL":"https:\/\/doi.org\/10.1139\/y91-028","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2,1]]}}}