{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T07:05:23Z","timestamp":1648537523413},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1992,9,1]],"date-time":"1992-09-01T00:00:00Z","timestamp":715305600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1992,9,1]]},"abstract":"<jats:p> In this study, the role of elevation of intracellular Ca<jats:sup>2+<\/jats:sup> and activation of protein kinase C on adrenergic-stimulated cyclic nucleotide accumulation and melatonin synthesis in rat pinealocytes was investigated. It was found that whereas KCl, ionomycin, and ouabain, three Ca<jats:sup>2+<\/jats:sup>-elevating agents, had a potentiating effect on adrenergic-stimulated cylic AMP response, their effects on melatonin synthesis were inhibitory. Similar inhibition was also observed when dibutyryl cyclic AMP was used to stimulate melatonin synthesis. By determining intracellular Ca<jats:sup>2+<\/jats:sup> directly, it was found that the enhancing effects of these agents on the cyclic AMP response but not their inhibitory effects on melatonin synthesis paralleled their abilities to elevate intracellular Ca<jats:sup>2+<\/jats:sup>. In comparison, activation of protein kinase C significantly enhanced the adrenergic-stimulated cyclic AMP response and, to a lesser degree, the adrenergic-stimulated N-acetyltransferase and melatonin levels. These results indicate that (i) Ca<jats:sup>2+<\/jats:sup>-elevating agents have opposite effects on adrenergic-stimulated cyclic AMP and melatonin production; (ii) a post cyclic AMP event of importance to melatonin synthesis is inhibited by these agents; and (iii) the mechanism of inhibition may not be directly related to their effect on intracellular Ca<jats:sup>2+<\/jats:sup>.Key words: intracellular calcium, protein kinase C, melatonin, pineal gland. <\/jats:p>","DOI":"10.1139\/y92-174","type":"journal-article","created":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T21:03:24Z","timestamp":1303592604000},"page":"1254-1260","source":"Crossref","is-referenced-by-count":1,"title":["Differential effects of intracellular calcium elevating agents on adrenergic-stimulated cyclic nucleotide and melatonin synthesis in rat pinealocytes"],"prefix":"10.1139","volume":"70","author":[{"given":"Anthony K.","family":"Ho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Girard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y92-174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:22:52Z","timestamp":1578237772000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y92-174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1992,9,1]]}},"alternative-id":["10.1139\/y92-174"],"URL":"https:\/\/doi.org\/10.1139\/y92-174","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,9,1]]}}}