{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:18:16Z","timestamp":1758925096340},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"S1","license":[{"start":{"date-parts":[[1992,5,15]],"date-time":"1992-05-15T00:00:00Z","timestamp":705888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1992,5,15]]},"abstract":"<jats:p> Spatial and temporal changes in the intracellular free Ca<jats:sup>2+<\/jats:sup> concentration in response to Ca<jats:sup>2+<\/jats:sup> influx at the cell membrane and to Ca<jats:sup>2+<\/jats:sup> release from intracellular organelles were studied by recording fluorescence of Ca<jats:sup>2+<\/jats:sup>-sensitive probes, fura 2 or indo 1, with conventional epifluorescence or confocal laser-scanning microscopy combined with recordings of Ca<jats:sup>2+<\/jats:sup>-dependent membrane responses in bullfrog sympathetic ganglion cells. It was found that an increase in the intracellular Ca<jats:sup>2+<\/jats:sup> induced by (an) action potential(s) in freshly isolated ganglion cells bathed in Ringer's solution was solely a result of Ca<jats:sup>2+<\/jats:sup> influx, while a rise in the intracellular Ca<jats:sup>2+<\/jats:sup> by Ca<jats:sup>2+<\/jats:sup> current in voltage-clamped cultured neurones was caused by not only Ca<jats:sup>2+<\/jats:sup> influx but also Ca<jats:sup>2+<\/jats:sup> release. This Ca<jats:sup>2+<\/jats:sup> release was suggested to occur by a voltage-dependent (and graded) mode of activation of a Ca<jats:sup>2+<\/jats:sup>-induced Ca<jats:sup>2+<\/jats:sup> release mechanism, explaining the lack of Ca<jats:sup>2+<\/jats:sup> release by action potentials (because of their short-lasting depolarization) in freshly isolated neurones. In both cases, there was an inward spread of an increase in intracellular Ca<jats:sup>2+<\/jats:sup>. On the other hand, all or nothing activation of Ca<jats:sup>2+<\/jats:sup>-induced Ca<jats:sup>2+<\/jats:sup> release occurred in the presence of caffeine, leading to the oscillation of Ca<jats:sup>2+<\/jats:sup> in the cells. Characteristics of this mode of Ca<jats:sup>2+<\/jats:sup> release and unique properties of drugs to block Ca<jats:sup>2+<\/jats:sup> release were described. Finally, the physiological significance of different types of Ca<jats:sup>2+<\/jats:sup> release was discussed.Key words: Ca<jats:sup>2+<\/jats:sup> current, Ca<jats:sup>2+<\/jats:sup>-induced Ca<jats:sup>2+<\/jats:sup> release, Ca<jats:sup>2+<\/jats:sup> transient, confocal microscope, bullfrog sympathetic ganglion cells. <\/jats:p>","DOI":"10.1139\/y92-245","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:59:07Z","timestamp":1303621147000},"page":"S64-S72","source":"Crossref","is-referenced-by-count":10,"title":["Intracellular Ca<sup>2+<\/sup> dynamics in response to Ca<sup>2+<\/sup> influx and Ca<sup>2+<\/sup> release in autonomic neurones"],"prefix":"10.1139","volume":"70","author":[{"given":"Kenji","family":"Kuba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Nohmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-Ying","family":"Hua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y92-245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T10:22:01Z","timestamp":1578219721000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y92-245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5,15]]},"references-count":0,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[1992,5,15]]}},"alternative-id":["10.1139\/y92-245"],"URL":"https:\/\/doi.org\/10.1139\/y92-245","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5,15]]}}}