{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:48:11Z","timestamp":1693219691632},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[1993,7,1]],"date-time":"1993-07-01T00:00:00Z","timestamp":741484800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:p> We have previously shown that acute insulin-induced normalization of glycemia in alloxan-diabetic (A-D) dogs results in marked inhibition of total pancreatic glucagon content, but normalization of somatostatin content. We suggested that this glucagon deficiency might account for A-cell unresponsiveness in diabetes. To examine these changes in detail at the islet level, morphometric and immunologic analyses were carried out on pancreata from four normal (N), four hyperglycemic A-D dogs (HD), and four A-D dogs after acute normalization of glycemia with insulin (ND). The total number of islets per pancreas (3.9\u2002\u00d7\u200210<jats:sup>6<\/jats:sup>\u2002\u00b1\u20020.5\u2002\u00d7\u200210<jats:sup>6<\/jats:sup>; determined from the number of islets per square millimetre) was reduced by 60% (p\u2002&lt;\u20020.001) in HD, and this was not affected by acute normalization of glycemia. Insulin content per islet was 1247\u2002\u00b1\u2002205\u2002pg in N, and this was reduced in both HD and ND to 2 and 5%, respectively (p\u2002&lt;\u20020.001). Similarly, insulin-containing B-cell area was 76\u2002\u00b1\u20021% of the total islet area in N, and was unmeasurable in HD and ND. Glucagon content per islet was 89\u2002\u00b1\u20026\u2002pg in N, and this was increased by 215% (p\u2002&lt;\u20020.001) in HD, but was normalized in ND. The A-cell area increased concomitantly by 170% from 17\u2002\u00b11 to 46\u2002\u00b1\u20022% (p\u2002&lt;\u20020.01) of islet area in HD, and remained elevated in ND. In contrast, somatostatin content per islet was 3.0\u2002\u00b1\u20021.1\u2002pg in N, but was increased by 567% (p\u2002&lt;\u20020.01) in HD and fell to 200% (p\u2002=\u2002ns) of N in ND. D-cell area increased by 55% in HD, from 11.5\u2002\u00b1\u20020.8 to 17.8\u2002\u00b1\u20020.9% (p\u2002&lt;\u20020.01), and remained elevated at 15.6\u2002\u00b1\u20021.1% (p\u2002&lt;\u20020.01) in ND. The ratio of somatostatin to glucagon content in the islet was therefore 0.03\u2002\u00b1\u20020.01 in N, and this was increased by 167 (p\u2002=\u2002ns) and 333% (p\u2002&lt;\u20020.05) in HD and ND, respectively. We speculate that the increase in the islet somatostatin to glucagon ratio in normoglycemic diabetic dogs may play a role in the suppression of glucagon responses in diabetes.Key words: islet, glucagon, somatostatin, diabetes, insulin-induced euglycemia. <\/jats:p>","DOI":"10.1139\/y93-074","type":"journal-article","created":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T21:03:28Z","timestamp":1303592608000},"page":"512-517","source":"Crossref","is-referenced-by-count":3,"title":["Increase in somatostatin to glucagon ratio in islets of alloxan-diabetic dogs: effect of insulin-induced euglycemia"],"prefix":"10.1139","volume":"71","author":[{"given":"K. S.","family":"Rastogi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. L.","family":"Brubaker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kawasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Efendic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Vranic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y93-074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:20:38Z","timestamp":1578237638000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y93-074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1993,7,1]]}},"alternative-id":["10.1139\/y93-074"],"URL":"https:\/\/doi.org\/10.1139\/y93-074","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,1]]}}}