{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:18:29Z","timestamp":1756991909291},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[1994,11,1]],"date-time":"1994-11-01T00:00:00Z","timestamp":783648000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1994,11,1]]},"abstract":"<jats:p> The factors that regulate the voltage-dependent Ca<jats:sup>2+<\/jats:sup> channels in pregnant uterine smooth muscle cells have not been elucidated, including any roles for protein kinase C (PKC). Therefore, the role of PKC in the regulation of the slow (L type) Ca<jats:sup>2+<\/jats:sup> channels was examined in myometrial cells isolated from late pregnant (18\u201319\u2002day) rat uterus, using the nystatin-perforated whole-cell voltage clamp. A PKC activator, phorbol 12, 13-dibutyrate (PDB), increased the L-type Ca<jats:sup>2+<\/jats:sup> current (I<jats:sub>Ca(L)<\/jats:sub>). Bath application of PDB (0.03 and 0.3\u2002\u03bcM) increased the peak amplitude of I<jats:sub>Ca(L)<\/jats:sub> by 21\u2002\u00b1\u200214% (n\u2002=\u20026) and 37\u2002\u00b1\u20028% (n\u2002=\u20029, p\u2002&lt;\u20020.01), respectively. PDB did not change the holding current or shift the current\u2013voltage relationship for I<jats:sub>Ca(L)<\/jats:sub>. The PKC inhibitors, H-7 (20\u2002\u03bcM) or staurosporine (10\u2002nM), reversed the effect of PDB. These results indicate that PKC may play a role in regulating Ca<jats:sup>2+<\/jats:sup> channel function in pregnant rat myometrial cells and, therefore, may be involved in control of uterine contraction.Key words: protein kinase C, phorbol ester, calcium current, myometrial cell, nystatin-perforated patch, whole-cell voltage clamp. <\/jats:p>","DOI":"10.1139\/y94-187","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:59:40Z","timestamp":1303635580000},"page":"1304-1307","source":"Crossref","is-referenced-by-count":18,"title":["Protein kinase C stimulates Ca<sup>2+<\/sup> current in pregnant rat myometrial cells"],"prefix":"10.1139","volume":"72","author":[{"given":"Keiichi","family":"Shimamura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masumi","family":"Kusaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Sperelakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y94-187","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:19:38Z","timestamp":1578237578000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y94-187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1994,11,1]]}},"alternative-id":["10.1139\/y94-187"],"URL":"https:\/\/doi.org\/10.1139\/y94-187","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,11,1]]}}}