{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:26:21Z","timestamp":1789986381566,"version":"4.0.1"},"reference-count":13,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[1995,7,1]],"date-time":"1995-07-01T00:00:00Z","timestamp":804556800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1995,7,1]]},"abstract":"<jats:p> High affinity amylin binding sites are present in the rat nucleus accumbens. These sites bind [<jats:sup>125<\/jats:sup>I]amylin with an affinity of 27\u2002pM and have high affinity for salmon calcitonin (sCT) and moderately high affinity for calcitonin gene related peptide (CGRP). N-terminally truncated peptides were tested for their ability to compete for [<jats:sup>125<\/jats:sup>I]amylin binding to these sites and to antagonize the metabolic and vascular actions of amylin. CGRP(8\u201337), sCT(8\u201332), and ac-[Asn<jats:sup>30<\/jats:sup>,Tyr<jats:sup>32<\/jats:sup>]sCT(8\u201332) (AC187) inhibited [<jats:sup>125<\/jats:sup>I]amylin binding to rat nucleus accumbens. Order of potency at inhibiting amylin binding (AC187 &gt; sCT(8\u201332) &gt; CGRP(8\u201337)) differed from the order of potency at inhibiting [<jats:sup>125<\/jats:sup>I]CGRP binding to SK-N-MC neuroblastoma cells (CGRP(8\u201337) &gt; AC187 &gt; sCT(8\u201332)). AC187 was the most potent antagonist of amylin's effects on isolated rat soleus muscle glycogen metabolism, and it was more effective than either sCT(8\u201332) or CGRP(8\u201337) at reducing amylin-stimulated hyperlactemia in rats. In contrast, CGRP(8\u201337) was the most potent peptide at antagonizing amylin-induced hypotension in rats. Amylin's hypotensive actions appear to be mediated by a weak action at CGRP receptors, while its metabolic actions are mediated by receptors with a distinct antagonist profile. AC187 is a potent antagonist of amylin binding sites in nucleus accumbens and of amylin's metabolic actions.Key words: amylin, calcitonin gene related peptide, diabetes, skeletal muscle, peptide receptors. <\/jats:p>","DOI":"10.1139\/y95-144","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T09:07:22Z","timestamp":1303636042000},"page":"1025-1029","source":"Crossref","is-referenced-by-count":32,"title":["Differential antagonism of amylin's metabolic and vascular actions with amylin receptor antagonists"],"prefix":"10.1139","volume":"73","author":[{"given":"K.","family":"Beaumont","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. X.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Pittner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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