{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:33:02Z","timestamp":1759962782048,"version":"3.38.0"},"reference-count":36,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[1995,11,1]],"date-time":"1995-11-01T00:00:00Z","timestamp":815184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup>plays a major role in vascular contraction, and a defect in intracellular Ca<jats:sup>2+<\/jats:sup>regulation has been associated with increased vascular reactivity in hypertension. To test the hypothesis that the sarcoplasmic reticulum does not adequately buffer Ca<jats:sup>2+<\/jats:sup>in deoxycorticosterone acetate (DOCA) hypertension, contractile experiments were performed with a specific inhibitor of the sarcoplasmic reticulum Ca<jats:sup>2+<\/jats:sup>ATPase, cyclopiazonic acid (CPA). Contractile force in aortic strips from DOCA and control rats was measured, using standard muscle bath procedures, to evaluate (i) Ca<jats:sup>2+<\/jats:sup>handling, assessing caffeine and serotonin (5HT) induced contractions in Ca<jats:sup>2+<\/jats:sup>-free buffer and (ii) relaxation rate after 5HT washout. Contractile responses elicited with 5HT (3\u2002\u00d7\u200210<jats:sup>\u22126<\/jats:sup>\u2002mol\/L) and caffeine (20\u2002mmol\/L) were greater in DOCA than in control arteries. CPA (1\u2002\u00d7\u200210<jats:sup>\u22127<\/jats:sup>to 3\u2002\u00d7\u200210<jats:sup>\u22125<\/jats:sup>\u2002mol\/L) reduced phasic contractions to 5HT and caffeine in DOCA and control aorta, and no differences in the IC<jats:sub>50<\/jats:sub>values were observed. Aortae from DOCA rats contracted when placed in normal buffer, subsequent to treatment with Ca<jats:sup>2+<\/jats:sup>-free buffer, but control aortae did not. CPA potentiated these responses in DOCA aorta and only caused a modest contraction in control aorta. CPA-induced contraction did not occur in Ca<jats:sup>2+<\/jats:sup>-free buffer, and it was inhibited by nifedipine (IC<jats:sub>50<\/jats:sub>\u2002=\u20024\u2002\u00d7\u200210<jats:sup>\u22129<\/jats:sup>\u2002mol\/L). The relaxation rate, after 5HT washout (3\u2002\u00d7\u200210<jats:sup>\u22126<\/jats:sup>\u2002mol\/L), was increased in DOCA aorta (2.6\u2002\u00b1\u20020.3\u2002min) compared with control (1.7\u2002\u00b1\u20020.2\u2002min), and CPA (10<jats:sup>\u22125<\/jats:sup>\u2002mol\/L) increased the relaxation rate in both groups. The results support the hypothesis of defective Ca<jats:sup>2+<\/jats:sup>handling in DOCA hypertension. However, an increased Ca<jats:sup>2+<\/jats:sup>influx, and not a decreased buffering ability of the sarcoplasmic reticulum, contributes to the enhanced vascular reactivity observed in DOCA hypertension.Key words: vascular smooth muscle, intracellular calcium mobilization, caffeine, cyclopiazonic acid, sarcoplasmic reticulum, deoxycorticosterone (DOCA) hypertension.<\/jats:p>","DOI":"10.1139\/y95-212","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T09:08:11Z","timestamp":1303636091000},"page":"1536-1545","source":"Crossref","is-referenced-by-count":17,"title":["Sarcoplasmic reticulum Ca<sup>2+<\/sup>uptake is not decreased in aorta from deoxycorticosterone acetate hypertensive rats: functional assessment with cyclopiazonic acid"],"prefix":"10.1139","volume":"73","author":[{"given":"Rita C. 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