{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T06:35:33Z","timestamp":1693377333781},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[1970,2,1]],"date-time":"1970-02-01T00:00:00Z","timestamp":2678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1970,2,1]]},"abstract":"<jats:p> Coxiella burnetii (Cb) in phase I (Ph I) multiplies very poorly in L-cells as shown by light and electron microscopy and infectivity titrations; Cb antigens, however, are detectable by immunofluorescence in most cells. Phase II (Ph II) organisms, in contrast, multiply well and produce typical particles in well-defined cytoplasmic vacuoles. Density gradient centrifugation has been applied to test the heterogeneity of the animal-maintained Nine Mile strain in Ph I and the corresponding egg-grown strain in Ph II. The evidence indicates that both strains are mixtures of Ph I and Ph II organisms, although they reacted as Ph I or Ph II antigens in the complement fixation test. The interaction of Ph I and Ph II organisms was studied in mixed infections in L-cells; suppression, or a less marked enhancement, of the growth of Coxiella burnetii was observed, depending on the conditions. It is suggested that Ph I and Ph II rickettsiae represent genetically different but closely related organisms, Ph I being the \"parent\" strain and Ph II the \"mutant\" strain; \"phase variation\" of Coxiella burnetii results from a selection of the mutant in the population under the prevailing environmental conditions. <\/jats:p>","DOI":"10.1139\/m70-021","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T16:24:16Z","timestamp":1265646256000},"page":"125-133","source":"Crossref","is-referenced-by-count":7,"title":["The interaction of <i>Coxiella burnetii<\/i> phase I and phase II in Earle's cells"],"prefix":"10.1139","volume":"16","author":[{"given":"Nonna","family":"Kordov\u00e1","sequence":"first","affiliation":[]},{"given":"Paul R.","family":"Burton","sequence":"additional","affiliation":[]},{"given":"Cora M.","family":"Downs","sequence":"additional","affiliation":[]},{"given":"David","family":"Paretsky","sequence":"additional","affiliation":[]},{"given":"Elena","family":"Kov\u00e1\u010dov\u00e1","sequence":"additional","affiliation":[]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m70-021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T06:17:21Z","timestamp":1578291441000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m70-021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1970,2,1]]}},"alternative-id":["10.1139\/m70-021"],"URL":"https:\/\/doi.org\/10.1139\/m70-021","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,2,1]]}}}