{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T03:17:03Z","timestamp":1715656623065},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[1972,6,1]],"date-time":"1972-06-01T00:00:00Z","timestamp":76204800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1972,6,1]]},"abstract":"<jats:p> The site of the cell barrier to actinomycin-D uptake was studied using a wild-type Escherichia coli strain P and its cell envelope-defective filamentous mutants, strains 6\u03b3 and 12\u03b3, both of which 'leak' \u03b2-galactosidase and alkaline phosphatase into the medium during growth indicating both membrane and cell-wall defects. Actinomycin-D entered the cells of these two mutant strains as evidenced by the inhibition of both <jats:sup>14<\/jats:sup>C-uracil incorporation and synthesis of the induced \u03b2-galactosidase system. Under similar conditions, no inhibition occurred in the wild-type strain and its sucrose-lysozyme prepared spheroplasts. Actinomycin-D did, however, inhibit the above-mentioned systems in the wild-type sucrose-lysozyme spheroplasts prepared in the presence of 2\u2002mM EDTA. The experimental data indicate that although the cell wall may act as a primary barrier or sieve to actinomycin-D, the cytoplasmic membrane should be considered the final and determinative barrier to this antibiotic. <\/jats:p>","DOI":"10.1139\/m72-139","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T11:50:08Z","timestamp":1265629808000},"page":"909-915","source":"Crossref","is-referenced-by-count":15,"title":["Sensitivity of normal and mutant strains of <i>Escherichia coli<\/i> to actinomycin-D"],"prefix":"10.1139","volume":"18","author":[{"given":"A. P.","family":"Singh","sequence":"first","affiliation":[]},{"given":"K.-J.","family":"Cheng","sequence":"additional","affiliation":[]},{"given":"J. W.","family":"Costerton","sequence":"additional","affiliation":[]},{"given":"E. S.","family":"Idziak","sequence":"additional","affiliation":[]},{"given":"J. M.","family":"Ingram","sequence":"additional","affiliation":[]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m72-139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:05:29Z","timestamp":1578269129000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m72-139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1972,6,1]]}},"alternative-id":["10.1139\/m72-139"],"URL":"https:\/\/doi.org\/10.1139\/m72-139","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,6,1]]}}}