{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:43:02Z","timestamp":1778632982563,"version":"3.51.4"},"reference-count":12,"publisher":"Canadian Science Publishing","issue":"13","license":[{"start":{"date-parts":[[1995,12,15]],"date-time":"1995-12-15T00:00:00Z","timestamp":818985600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1995,12,15]]},"abstract":"<jats:p>Poly(hydroxyalkanoates) (PHAs) were isolated from Pseudomonas aeruginosa 44T1 cultivated on euphorbia oil and castor oil. With the aid of 2-D proton NMR spectra and proton-detected multiple bond coherence NMR spectra the structures of the PHAs were determined. In addition to the usual PHA constituents (C<jats:sub>6<\/jats:sub>\u2013C<jats:sub>14<\/jats:sub>3-hydroxy fatty acids), PHAs formed from euphorbia oil contained \u03948,9-epoxy-3-hydroxy-5c-tetradecenoate, and probably \u03946,7-epoxy-3-hydroxydodecanoate and \u03944,5-epoxy-3-hydroxydecanoate. These novel constituents account for approximately 15% of the total amount of monomers and are clearly generated via \u03b2-oxidation of vernolic acid (\u039412,13-epoxy-9c-octadecenoic acid), the main component of euphorbia oil. In PHAs formed from castor oil, 7% of the monomers found were derived from ricinoleic acid (12-hydroxy-9c-octadecenoic acid). The presence of 3,8-dihydroxy-5c tetradecenoate was clearly demonstrated. Furthermore, NMR analysis strongly suggested the presence of 3,6-dihydroxydodecanoate, 6-hydroxy-3c-dodecenoate, and 4-hydroxydecanoate.Key words: poly(hydroxyalkanoates), pseudomonads, ricinoleic acid, vernolic acid.<\/jats:p>","DOI":"10.1139\/m95-163","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T17:53:49Z","timestamp":1255024429000},"page":"14-21","source":"Crossref","is-referenced-by-count":69,"title":["Formation of novel poly(hydroxyalkanoates) from long-chain fatty acids"],"prefix":"10.1139","volume":"41","author":[{"given":"Gerrit","family":"Eggink","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter de","family":"Waard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gern N. M.","family":"Huijberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/BF00169901","volume":"35","author":"Andres C.","year":"1991","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"p_2\/p_2_1","first-page":"355","volume":"65","author":"Bax A.","year":"1985","journal-title":"J. Magn. Reson."},{"key":"p_3\/p_3_1","doi-asserted-by":"crossref","first-page":"7926","DOI":"10.1021\/ja00231a081","volume":"110","author":"Bax A.","year":"1988","journal-title":"J. Am. Chem. Soc."},{"key":"p_4\/p_4_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0021-9258(18)54151-X","volume":"268","author":"de Waard P.","year":"1993","journal-title":"J. Biol. Chem."},{"key":"p_5\/p_5_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/j.1574-6968.1992.tb05833.x","volume":"103","author":"Eggink G.","year":"1992","journal-title":"FEMS Microbiol. Rev."},{"key":"p_6\/p_6_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0926-6690(92)90014-M","volume":"1","author":"Eggink G.","year":"1992","journal-title":"Ind. Crops Prod."},{"key":"p_7\/p_7_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1438-8677.1991.tb00223.x","volume":"104","author":"Gerblin H.","year":"1991","journal-title":"Bot. Acta"},{"key":"p_8\/p_8_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/AEM.58.2.536-544.1992","volume":"58","author":"Huijberts G.N.M.","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"p_9\/p_9_1","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1128\/JB.176.6.1661-1666.1994","volume":"176","author":"Huijberts G.N.M.","year":"1994","journal-title":"J. Bacteriol."},{"key":"p_10\/p_10_1","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1021\/ma00033a002","volume":"25","author":"Kim Y.B.","year":"1992","journal-title":"Macromolecules"},{"key":"p_11\/p_11_1","doi-asserted-by":"crossref","first-page":"2924","DOI":"10.1128\/AEM.54.12.2924-2932.1988","volume":"54","author":"Lageveen R.G.","year":"1988","journal-title":"Appl. Environ. Microbiol."},{"key":"p_12\/p_12_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0926-6690(92)90046-X","volume":"1","author":"Muuse B.G.","year":"1992","journal-title":"Ind. Crops Prod."}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m95-163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T01:00:35Z","timestamp":1634000435000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m95-163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12,15]]},"references-count":12,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1995,12,15]]}},"alternative-id":["10.1139\/m95-163"],"URL":"https:\/\/doi.org\/10.1139\/m95-163","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12,15]]}}}