{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:26:35Z","timestamp":1756992395817},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1968,5,1]],"date-time":"1968-05-01T00:00:00Z","timestamp":-52704000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1968,5,1]]},"abstract":"<jats:p> Application of the standard methods for the preparation of fraction I protein (ribulosediphosphate carboxylase) from spinach leaves gives low yields when applied to other species. We describe here simple methods for the preparation of pure fraction I protein in good yield from spinach, peas, beans, and Chlorella. The electrophoretic mobilities (on polyacrylamide electrophoretograms) of bean and Chlorella fraction I differ from each other and from the mobilities of pea and spinach protein. <\/jats:p>","DOI":"10.1139\/o68-066","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T22:09:40Z","timestamp":1261087780000},"page":"441-444","source":"Crossref","is-referenced-by-count":13,"title":["A simple method for the isolation of fraction I protein of chloroplasts"],"prefix":"10.1139","volume":"46","author":[{"given":"J. H.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. R.","family":"McCalla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o68-066","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:08:29Z","timestamp":1578269309000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o68-066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1968,5,1]]}},"alternative-id":["10.1139\/o68-066"],"URL":"https:\/\/doi.org\/10.1139\/o68-066","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1968,5,1]]}}}