{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T18:54:23Z","timestamp":1758394463007},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1976,9,1]],"date-time":"1976-09-01T00:00:00Z","timestamp":210384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1976,9,1]]},"abstract":"<jats:p> Glycopeptides were prepared from the delipidized protein of low-density lipoprotein (LDL, d\u2002=\u20021.019\u20131.063) of three normal and three familial heterozygous type II hyperlipoproteinemic (HLP) subjects. The glycopeptides of all subjects were resolved into three groups by gel filtration on Bio-Gel P6 following papain (EC 3.4.22.2) digestion and initial purification on Bio-Gel P2. In normal individuals the component of largest molecular weight (F-1) contained mannose (Man), N-acetyl glucosamine (GlcNAc), galactose (Gal), and N-acetyl neuraminic acid (NANA) in the respective amounts of 45.9\u2002\u00b1\u20026.7, 37.3\u2002\u00b1\u20025.9, 28.6\u2002\u00b1\u20023.4, and 27.0\u2002\u00b1\u20023.9\u2002nmol\/mg original apoprotein. The group of smallest molecular weight (F-3) contained essentially only Man (25.8\u2002\u00b1\u20021.5\u2002nmol\/mg protein) and GlcNac (3.0\u2002\u00b1\u20020.4\u2002nmol\/mg protein) with traces of Gal and NANA. A group of intermediate molecular weight (F-2) exhibited considerable heterogeneity and contained Man, GlcNAc, Gal, and NANA in the amounts of45.9\u2002\u00b1\u20025.1, 18.3\u2002\u00b1\u20021.7, 11.0\u2002\u00b1\u20021.7, and 7.7\u2002\u00b1\u20021.2\u2002nmol\/mg protein. While the major portion of NANA (78%), Gal (71%), and GlcNAc (64%) was present in F-1, approximately 22% of the total Man was in F-3. No major differences were detected in the carbohydrate composition of the three glycopeptide fractions of LDL apoprotein from normal and Type II subjects. <\/jats:p>","DOI":"10.1139\/o76-119","type":"journal-article","created":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T16:17:56Z","timestamp":1261153076000},"page":"829-833","source":"Crossref","is-referenced-by-count":12,"title":["Isolation and carbohydrate composition of glycopeptides of human apo low-density lipoprotein from normal and type II hyperlipoproteinemic subjects"],"prefix":"10.1139","volume":"54","author":[{"given":"P.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Carl","family":"Breckenridge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o76-119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:52:30Z","timestamp":1578246750000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o76-119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1976,9,1]]}},"alternative-id":["10.1139\/o76-119"],"URL":"https:\/\/doi.org\/10.1139\/o76-119","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1976,9,1]]}}}