{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:03:47Z","timestamp":1761059027117},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[1978,6,1]],"date-time":"1978-06-01T00:00:00Z","timestamp":265507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1978,6,1]]},"abstract":"<jats:p> The recently discovered heat-stable inhibitor protein of the Ca<jats:sup>2+<\/jats:sup>-activated cyclic nucleotide phosphodiesterase (Sharma, R, K., Wirch, E. &amp;Warg, J. H. (1978) J. Biol. Chem., in press) has been purified 238\u2002214-fold from bovine brain extract using an affinity column of the modulator protein \u2013 Sepharose 4B conjugate. The purified sample appears to be homogeneous as judged by sodium dodecyl sulphate (SDS) gel electrophoresis. The protein band has a mobility corresponding to that of a polypeptide of molecular weight 68\u2002000. Since the heat-stable inhibitor protein has a molecular weight of 70\u2002000 under nondenaturing conditions, it suggests that it is a monomeric protein. The protein has no inhibitory activity toward the cAMP-dependent protein kinase or protein phosphatase. The purified sample has been tested for various enzyme activities which include ATPase, GTPase, cAMP phosphodiesterase, cGMP phosphodiesterase, 5\u2032-nucleotidase, and protein kinase. None of these activities are exhibited by the purified sample. <\/jats:p>","DOI":"10.1139\/o78-090","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T09:53:24Z","timestamp":1261389204000},"page":"598-604","source":"Crossref","is-referenced-by-count":33,"title":["Purification of the heat-stable inhibitor protein of the Ca<sup>2+<\/sup>-activated cyclic nucleotide phosphodiesterase by affinity chromatography"],"prefix":"10.1139","volume":"56","author":[{"given":"Rajendra K.","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rekha","family":"Desai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry H.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o78-090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:53:11Z","timestamp":1578246791000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o78-090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1978,6,1]]}},"alternative-id":["10.1139\/o78-090"],"URL":"https:\/\/doi.org\/10.1139\/o78-090","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1978,6,1]]}}}