{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:19:59Z","timestamp":1760897999313},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[1983,12,1]],"date-time":"1983-12-01T00:00:00Z","timestamp":439084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem. Cell Biol."],"published-print":{"date-parts":[[1983,12,1]]},"abstract":"<jats:p> A hybrid Escherichia coli: Col E1 plasmid, pLC36-19, containing a catalase gene has been identified in the Clarke and Carbon colony bank. Catalase activity was amplified two- to three-fold in the pLC36-19-containing strain relative to other hybrid-plasmid-containing strains and this activity could be induced three- or four-fold by hydrogen peroxide or ascorbic acid. The plasmid was transferred to a strain chromosomally deficient in catalase synthesis, resulting in a strain with high and inducible levels of catalase. The plasmid was also transferred to a minicell-producing strain and minicells harbouring the plasmid were found to synthesize a labelled protein with a molecular weight of 84\u2002000 characteristic of catalase from E. coli. A catalase activity was also synthesized by the plasmid-containing minicells. Two catalase activities with associated peroxidase activities coded for by the plasmid were separable by polyacrylamide gel electrophoresis and migrated coincident with chromosomally encoded catalase\u2013peroxidase activities. A third catalase activity which did not have an associated peroxidase activity was not coded for by the plasmid. A physical map of the 25.5-kilobase pair plasmid was constructed by restriction nuclease analysis and the relative positions of 38 restriction sites were defined. <\/jats:p>","DOI":"10.1139\/o83-168","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T13:59:26Z","timestamp":1261576766000},"page":"1315-1321","source":"Crossref","is-referenced-by-count":18,"title":["Identification and physical characterization of a Col E1 hybrid plasmid containing a catalase gene of <i>Escherichia coli<\/i>"],"prefix":"10.1139","volume":"61","author":[{"given":"Peter C.","family":"Loewen","sequence":"first","affiliation":[]},{"given":"Barbara L.","family":"Triggs","sequence":"additional","affiliation":[]},{"given":"Glen R.","family":"Klassen","sequence":"additional","affiliation":[]},{"given":"Joel H.","family":"Weiner","sequence":"additional","affiliation":[]}],"member":"155","container-title":["Canadian Journal of Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o83-168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T15:39:19Z","timestamp":1578238759000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o83-168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1983,12,1]]}},"alternative-id":["10.1139\/o83-168"],"URL":"https:\/\/doi.org\/10.1139\/o83-168","relation":{},"ISSN":["0714-7511"],"issn-type":[{"value":"0714-7511","type":"print"}],"subject":[],"published":{"date-parts":[[1983,12,1]]}}}