{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T10:38:48Z","timestamp":1761561528425},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1981,8,1]],"date-time":"1981-08-01T00:00:00Z","timestamp":365472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1981,8,1]]},"abstract":"<jats:p> Single ventricular cells have been isolated from adult guinea pig heart using a collagenase enzyme method. The procedure is straightforward, takes about 20\u2002min, and yields 40\u201365% rod-shaped cells which are quiescent in millimolar Ca<jats:sup>2+<\/jats:sup> solution. Electrophysiological studies involving external ion changes and ionic channel inhibitors suggest that the cells retain their active membrane properties following this enzymatic dissociation. <\/jats:p>","DOI":"10.1139\/y81-139","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:52:07Z","timestamp":1303620727000},"page":"907-910","source":"Crossref","is-referenced-by-count":27,"title":["Single cells from adult mammalian heart: Isolation procedure and preliminary electrophysiological studies"],"prefix":"10.1139","volume":"59","author":[{"given":"J. Omar","family":"Bustamante","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshifumi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terence F.","family":"McDonald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y81-139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:24:18Z","timestamp":1578223458000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y81-139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1981,8,1]]}},"alternative-id":["10.1139\/y81-139"],"URL":"https:\/\/doi.org\/10.1139\/y81-139","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"type":"print","value":"0008-4212"},{"type":"electronic","value":"1205-7541"}],"subject":[],"published":{"date-parts":[[1981,8,1]]}}}