{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:19:52Z","timestamp":1771625992310,"version":"3.50.1"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1982,3,1]],"date-time":"1982-03-01T00:00:00Z","timestamp":383788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1982,3,1]]},"abstract":"<jats:p> Neurones from brain and spinal cord of foetal mice were grown dissociated in monolayer cultures for 4\u20136 weeks prior to electropharmacological analysis. Neurones were immersed in a Hanks balanced salt solution while drugs and ions were applied by pressure microperfusion during intracellular recordings obtained by conventional techniques. L-Glutamate and its analogues, L-aspartate, DL-homocysteate, N-methyl-D-aspartate, and DL-ibotenate activated two distinct mechanisms of excitation. The primary effect was depolarization accomplished by an apparent decrease of neurone input conductance (G<jats:sub>m<\/jats:sub>). However, in most instances an expected increase in G<jats:sub>m<\/jats:sub> was also observed, especially if membrane potential was reduced by tonic depolarization. Another glutamate analogue, DL-kainate, never decreased G<jats:sub>m<\/jats:sub> and invariably increased G<jats:sub>m<\/jats:sub> at all membrane potentials tested. The decrease of G<jats:sub>m<\/jats:sub> evoked by glutamate and related compounds was strongly dependent upon membrane potential. It was most pronounced at potentials near resting values (\u221240 to \u221260\u2002mV) and diminished both with depolarization or hyperpolarization from this range. This apparent decrease favoured the electrogenesis of regenerative potentials that were insensitive to tetrodotoxin. A voltage-dependent increase in sodium and(or) calcium conductance (G<jats:sub>Na<\/jats:sub>, G<jats:sub>Ca<\/jats:sub>) or a decrease in potassium conductance (G<jats:sub>K<\/jats:sub>) is suggested to account for this decrease in G<jats:sub>m<\/jats:sub>. Divalent cations (Mg and Co) reduced the depolarizing actions of all amino acids except for those to kainate. The decrease in G<jats:sub>m<\/jats:sub> was more sensitive to Mg than was the increase of G<jats:sub>m<\/jats:sub>. However, the receptor antagonist DL-\u03b1-aminoadipate blocked both changes in conductance and responses to all amino acids with the exception of those to kainate. The possible existence of multiple receptors for glutamate is also discussed. <\/jats:p>","DOI":"10.1139\/y82-039","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:52:18Z","timestamp":1303620738000},"page":"282-296","source":"Crossref","is-referenced-by-count":69,"title":["The effects of <scp>L<\/scp>-glutamate and its analogues upon the membrane conductance of central murine neurones in culture"],"prefix":"10.1139","volume":"60","author":[{"given":"J. F.","family":"MacDonald","sequence":"first","affiliation":[]},{"given":"J. M.","family":"Wojtowicz","sequence":"additional","affiliation":[]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y82-039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:24:08Z","timestamp":1578223448000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y82-039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,3,1]]}},"alternative-id":["10.1139\/y82-039"],"URL":"https:\/\/doi.org\/10.1139\/y82-039","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3,1]]}}}