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We aimed to test the influence of phenolic acids on glucose and glutamine cellular uptake by a breast (MCF-7) and a pancreatic (AsPC-1) cancer cell line. Several phenolic acids (caffeic, ferrulic, proctocatechuic, coumaric and gallic acid) affected <jats:sup>3<\/jats:sup>H-glutamine and\/or <jats:sup>3<\/jats:sup>H-deoxy-d-glucose (<jats:sup>3<\/jats:sup>H-DG) uptake. Gallic acid (100\u00a0\u00b5M) caused a 3-fold increase in <jats:sup>3<\/jats:sup>H-DG uptake by AsPC-1 cells, associated with a 3.7-fold increase in lactic acid production. Gallic acid stimulated GLUT1-mediated <jats:sup>3<\/jats:sup>H-DG uptake and increased the affinity of this transporter for <jats:sup>3<\/jats:sup>H-DG. We further verified that gallic acid does not change GLUT1 transcription rates and cellular redox state and that its effect does not involve PI3K, mTOR and MAP kinases and is not associated with a proproliferative effect. Gallic acid also increased <jats:sup>3<\/jats:sup>H-DG uptake by MCF-7 cells, although less potently. Further investigation is necessary to elucidate the cellular pathways involved in this effect of gallic acid. <\/jats:p>","DOI":"10.1139\/cjpp-2022-0260","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T12:00:20Z","timestamp":1674475220000},"page":"90-105","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Gallic acid markedly stimulates GLUT1-mediated glucose uptake by the AsPC-1 pancreatic cancer cell line"],"prefix":"10.1139","volume":"101","author":[{"given":"S\u00edlvia M.","family":"Torres","sequence":"first","affiliation":[{"name":"Faculty of Sciences, University of Porto","place":["Porto, Portugal"]}]},{"given":"Francisca P.","family":"Carmo","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Porto","place":["Porto, Portugal"]},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, University of Porto","place":["Porto, 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