{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:40:33Z","timestamp":1773726033584,"version":"3.50.1"},"reference-count":58,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p> The Sahara desert is the largest warm desert in the world and a poorly explored area. Small water-bodies occur across the desert and are crucial habitats for vertebrate biodiversity. Environmental DNA (eDNA) is a powerful tool for species detection and is being increasingly used to conduct biodiversity assessments. However, there are a number of difficulties with sampling eDNA from such turbid water-bodies and it is often not feasible to rely on electrical tools in remote desert environments. We trialled a manually powered filtering method in Mauritania, using pre-filtration to circumvent problems posed by turbid water in remote arid areas. From nine vertebrate species expected in the water-bodies, four were detected visually, two via metabarcoding, and one via both methods. Difficulties filtering turbid water led to severe constraints, limiting the sampling protocol to only one sampling point per study site, which alone may largely explain why many of the expected vertebrate species were not detected. The amplification of human DNA using general vertebrate primers is also likely to have contributed to the low number of taxa identified. Here we highlight a number of challenges that need to be overcome to successfully conduct metabarcoding eDNA studies for vertebrates in desert environments in Africa. <\/jats:p>","DOI":"10.1139\/gen-2018-0071","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T21:35:53Z","timestamp":1539380153000},"page":"807-814","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA"],"prefix":"10.1139","volume":"61","author":[{"given":"Bastian","family":"Egeter","sequence":"first","affiliation":[{"name":"CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos da Universidade do Porto, Instituto de Ci\u00eancias Agr\u00e1rias de Vair\u00e3o, R. Padre Armando Quintas 7, 4485-661, Vair\u00e3o, Portugal."}]},{"given":"Sara","family":"Peixoto","sequence":"additional","affiliation":[{"name":"CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos da Universidade do Porto, Instituto de Ci\u00eancias Agr\u00e1rias de Vair\u00e3o, R. Padre Armando Quintas 7, 4485-661, Vair\u00e3o, Portugal."},{"name":"Departamento de Biologia da Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal."}]},{"given":"Jos\u00e9 C.","family":"Brito","sequence":"additional","affiliation":[{"name":"CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos da Universidade do Porto, Instituto de Ci\u00eancias Agr\u00e1rias de Vair\u00e3o, R. Padre Armando Quintas 7, 4485-661, Vair\u00e3o, Portugal."},{"name":"Departamento de Biologia da Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal."}]},{"given":"Simon","family":"Jarman","sequence":"additional","affiliation":[{"name":"Trace and Environmental DNA (TrEnD) Laboratory, Molecular and Life Sciences, Curtin University, WA, 6102, Australia."},{"name":"Environomics Future Science Platform, CSIRO National Collections and Marine Infrastructure, Crawley, WA, 6009, Australia."}]},{"given":"Pamela","family":"Puppo","sequence":"additional","affiliation":[{"name":"CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos da Universidade do Porto, Instituto de Ci\u00eancias Agr\u00e1rias de Vair\u00e3o, R. 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