{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:16:15Z","timestamp":1761894975077},"reference-count":65,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p>The purpose of the study was to determine if paralytic shellfish poisoning (PSP) toxins are present in extracellular bacteria isolated from a toxic strain of the dinoflagellate Alexandrium minutum . A quantitative analysis was carried out of viable culturable bacteria attached to the surface of dinoflagellates and of bacteria present in dinoflagellate culture medium. A numerical taxonomy study was undertaken for presumptive identification of bacteria attached to the surface of dinoflagellates. Members of the following genera were detected on the cell surface of A. minutum: Cellulophaga , Marinomonas , Pseudoalteromonas , and Vibrio . The presence of intracellular PSP toxins in bacteria isolated from the cell surface of dinoflagellates was analysed by high-performance liquid chromatography with fluorescence detection (HPLC\u2013FLD). Compounds that eluted at the same time as the standards of the PSP toxins GTX-2, GTX-3, GTX-4, dcGTX-2, and dcGTX-3 were present in some of the bacterial cell extracts. Natural fluorescent bacterial compounds, coeluting with some PSP toxins, were also detected. The results obtained showed that the fluorescent compounds, identified as putative PSP toxins by HPLC\u2013FLD, did not correspond to any PSP analogue. This allowed us to reject the hypothesis that extracellular bacteria attached to the surface of dinoflagellates produce PSP toxins.<\/jats:p>","DOI":"10.1139\/w09-047","type":"journal-article","created":{"date-parts":[[2009,11,2]],"date-time":"2009-11-02T13:04:07Z","timestamp":1257167047000},"page":"943-954","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of the production of paralytic shellfish poisoning toxins by extracellular bacteria isolated from the toxic dinoflagellate<i>Alexandrium minutum<\/i>"],"prefix":"10.1139","volume":"55","author":[{"given":"Mar\u00eda Jes\u00fas","family":"Prol","sequence":"first","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"C\u00e1stor","family":"Guisande","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Aldo","family":"Barreiro","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Beatriz","family":"M\u00edguez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Pablo","family":"de la Iglesia","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Adriano","family":"Villar","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Ana","family":"Gago-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]},{"given":"Mar\u00eda Pilar","family":"Combarro","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolox\u00eda, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"\u00c1rea de Ecolox\u00eda, Departamento de Ecolox\u00eda e Biolox\u00eda Animal, Facultade de Biolox\u00eda, Universidade de Vigo, Vigo, Spain."},{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Qu\u00edmica, Universidade de Vigo, Vigo, Spain."}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1099\/00207713-33-3-599","volume":"33","author":"Allen D.A.","year":"1983","journal-title":"Int. J. Syst. Bacteriol."},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1017\/S0967026202003955"},{"key":"rg3\/ref3","unstructured":"Anderson, D.M. 1989. Toxic algal blooms and red tides: a global perspective.InBiology, environmental science and toxicology.Edited byT. Okaichi, D.M. Anderson, and T. Nemoto. Elsevier Science, New York. pp.\u00a011\u201316."},{"key":"rg4\/ref4","first-page":"24","volume":"1","author":"Arnott G.H.","year":"1998","journal-title":"Advancing Food Safety"},{"key":"rg5\/ref5","unstructured":"Babinchak, J.A., McGovern, E.R., and Doucette, G.J. 1998. Isolation and characterization of the bacterial flora associated with PSP-related dinoflagellate species.InHarmful algae.Edited byB. Reguera, J. Blanco, M.L. Fern\u00e1ndez, and T. Wyatt. Xunta de Galicia and IOC of UNESCO, Santiago de Compostela. pp. 410\u2013413."},{"key":"rg6\/ref6","unstructured":"Bain, N., and Shewan, J.M. 1968. Identification ofAeromonas,Vibrioand related microorganisms.InIdentification methods for microbiologists.Edited byB.M. Gibbs and D.A. Shapton. Academic Press, London, U.K. pp.\u00a079\u201384."},{"key":"rg7\/ref7","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1099\/00221287-30-3-409","volume":"30","author":"Baird-Parker A.C.","year":"1963","journal-title":"J. Gen. Microbiol."},{"key":"rg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(02)00314-8"},{"key":"rg9\/ref9","unstructured":"Baumman, P., and Baumman, L. 1981. The marine gram negative eubacteria: generaPhotobacterium,Beneckea,Alteromonas,PseudomonasandAlcaligenes.InThe prokaryotes. A handbook on habitats, isolation and identification of bacteria.Edited byM.P. Starr, H. Stolp, H.G. Tr\u00fcper, A. Balows, and H. Schlegel. Springer-Verlag, New York. pp.\u00a01302\u20131331."},{"key":"rg10\/ref10","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1128\/JB.107.1.268-294.1971","volume":"107","author":"Baumman P.","year":"1971","journal-title":"J. Bacteriol."},{"key":"rg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8817.2002.01045.x"},{"key":"rg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1080\/09670260010001735921"},{"key":"rg13\/ref13","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1128\/AEM.44.4.992-993.1982","volume":"44","author":"Buck J.D.","year":"1982","journal-title":"Appl. Environ. Microbiol."},{"key":"rg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1692-1697.2000"},{"key":"rg16\/ref16","unstructured":"Doucette, G.J., and Powell, C.L. 1998. Algal-bacterial interactions: can they determine the PSP-related toxicity of dinoflagellates?InHarmful algae.Edited byB. Reguera, J. Blanco, M.L. Fern\u00e1ndez, and T. Wyatt. Xunta de Galicia and IOC of UNESCO, Santiago de Compostela. pp.\u00a0406\u2013409."},{"key":"rg17\/ref17","unstructured":"Doucette, G.J., and Trick, C.G. 1995. Characterization of bacteria associated with different isolates ofAlexandrium tamarense.InHarmful marine algal blooms.Edited byP. Lassus, G. Arzul, E. Erard, P. Gentien, and C. Marcaillou. Lavoisier, Paris. pp.\u00a033\u201338."},{"key":"rg18\/ref18","unstructured":"Doucette, G.J., Kodama, G., Franca, S., and Gallacher, S. 1997. Bacterial interactions with harmful algal bloom species: bloom ecology, toxigenesis and cytology.InThe physical ecology of harmful algal blooms.Edited byD.M. Anderson, A.D. Cembella, and G.M. Hallegraeff. NATO\/ASI Series. Springer-Verlag, Heidelberg. pp.\u00a0619\u2013647."},{"key":"rg19\/ref19","unstructured":"Espinosa de los Monteros, J., and Labarta, U. 1988. Patolog\u00eda en acuicultura. Plan de formaci\u00f3n de t\u00e9cnicos superiores en acuicultura (FEUGA). Industrias graficas Espa\u00f1a S.L. Madrid."},{"key":"rg20\/ref20","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/S0723-2020(11)80432-7","volume":"18","author":"Esteve C.","year":"1995","journal-title":"Syst. Appl. Microbiol."},{"key":"rg21\/ref21","unstructured":"Franca, S., Viegas, S., Mascarenhas, V., Pinto, L., and Doucette, G.J. 1995. Prokaryotes in association with a toxicAlexandrium lusitanicumin culture.InHarmful marine algal blooms.Edited byP. Lassus, G. Arzul, E. Erard, P. Gentien, and C. Marcaillou. Lavoisier, Paris. pp.\u00a033\u201338."},{"key":"rg22\/ref22","unstructured":"Franca, S., Pinto, L., Alvito, P., Sousa, I., Vasconcelos, V., and Doucette, G.J. 1996. Studies of prokaryotes associated with PSP producing dinoflagellates.InHarmful and toxic algal blooms.Edited byT. Yasumoto, Y. Oshima, and Y. Fukuyo. IOC of UNESCO, Paris. pp.\u00a0347\u2013350."},{"key":"rg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1007\/BF02267925"},{"key":"rg24\/ref24","doi-asserted-by":"publisher","DOI":"10.3354\/meps203161"},{"key":"rg25\/ref25","unstructured":"Gallacher, S., Flynn, K.J., Leftley, J., Lewis, J., Munro, P.D., and Birkbeck, T.H. 1996. Bacterial production of sodium channel blocking toxins.InHarmful and toxic algal blooms.Edited byT. Yasumoto, Y. Oshima, and Y. Fukuyo. IOC of UNESCO, Paris. pp.\u00a0355\u2013358."},{"key":"rg26\/ref26","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/AEM.63.1.239-245.1997","volume":"63","author":"Gallacher S.","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"rg27\/ref27","doi-asserted-by":"crossref","first-page":"234","DOI":"10.2216\/i0031-8884-32-3-234.1","volume":"32","author":"Guillard R.R.L.","year":"1993","journal-title":"Phycologia"},{"key":"rg28\/ref28","doi-asserted-by":"publisher","DOI":"10.3354\/meps225169"},{"key":"rg29\/ref29","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2216\/i0031-8884-32-2-79.1","volume":"32","author":"Hallegraeff G.M.","year":"1993","journal-title":"Phycologia"},{"key":"rg30\/ref30","unstructured":"Hallegraeff, G.M. 1995. Harmful algal blooms: a global review.InManual on harmful marine microalgae. Vol. 33.Edited byG.M. Hallegraeff, D.M. Anderson,and A.D. Cembella. IOC Manuals and Guides. UNESCO, Paris. pp.\u00a01\u201322."},{"issue":"6","key":"rg31\/ref31","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1128\/AEM.55.6.1435-1446.1989","volume":"55","author":"Hansen G.H.","year":"1989","journal-title":"Appl. Environ. Microbiol."},{"key":"rg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(91)90049-V"},{"key":"rg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2001.tb00843.x"},{"key":"rg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2001.tb00864.x"},{"key":"rg35\/ref35","doi-asserted-by":"publisher","DOI":"10.3354\/meps280199"},{"key":"rg36\/ref36","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/MMBR.58.4.603-615.1994","volume":"58","author":"Kepner R.L.","year":"1994","journal-title":"Microbiol. Rev."},{"key":"rg37\/ref37","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1128\/AEM.29.3.414-421.1975","volume":"29","author":"Kitamikado M.","year":"1975","journal-title":"Appl. Microbiol."},{"key":"rg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1983.tb00402.x"},{"key":"rg39\/ref39","unstructured":"Kodama, M. 1990. Possible links between bacteria and toxin production in algal blooms.InToxic marine phytoplankton.Edited byE. Gran\u00e9li, B. Sundstr\u00f6m, L. Edler, and D.M. Anderson. Elsevier Science, New York. pp.\u00a052\u201361."},{"key":"rg40\/ref40","first-page":"1075","volume":"52","author":"Kodama M.","year":"1988","journal-title":"Agric. Biol. Chem."},{"key":"rg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1997.tb00442.x"},{"issue":"3","key":"rg42\/ref42","doi-asserted-by":"crossref","first-page":"280","DOI":"10.2216\/i0031-8884-40-3-280.1","volume":"40","author":"Lewis J.","year":"2001","journal-title":"Phycologia"},{"key":"rg43\/ref43","first-page":"241","volume":"31","author":"Lwoff A.","year":"1981","journal-title":"Int. J. Syst. Bacteriol."},{"key":"rg44\/ref44","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1099\/00207713-36-2-228","volume":"36","author":"Lwoff A.","year":"1986","journal-title":"Int. J. Syst. Bacteriol."},{"key":"rg45\/ref45","unstructured":"Maas, E.W., van Gennip, E., Truman, P., and Brooks, H.J.L. 1998. The effect of growth conditions on the production of sodium channel blockers by two marine bacterial isolates from toxic algal cultures.InHarmful algae.Edited byB. Reguera, J. Blanco, M.L. Fern\u00e1ndez, and T. Wyatt. Xunta de Galicia and IOC of UNESCO, Vigo, Spain. pp.\u00a0414\u2013416."},{"key":"rg46\/ref46","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5693-5698.2003"},{"key":"rg47\/ref47","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6496(03)00070-9"},{"key":"rg48\/ref48","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/S0723-2020(89)80080-3","volume":"12","author":"Ortigosa M.","year":"1989","journal-title":"Syst. Appl. Microbiol."},{"key":"rg49\/ref49","first-page":"453","volume":"13","author":"Ortigosa M.","year":"1997","journal-title":"Microbiologia"},{"key":"rg50\/ref50","unstructured":"Oshima, Y., Sugino, K., and Yasumoto, T. 1989. Latest advances in HPLC analysis of paralytic shellfish toxins.InMycotoxins and phycotoxins.Edited byS. Natori, K. Hashimoto, and Y. Ueno. Elsevier Science, Amsterdam, the Netherlands. pp.\u00a0319\u2013326."},{"key":"rg51\/ref51","unstructured":"Pascual, M. R., and Calder\u00f3n y Pascual, V. 2000. Microbiolog\u00eda alimentaria. Metodolog\u00eda anal\u00edtica para alimentos y bebidas. D\u00edaz de Santos S.A., Spain."},{"key":"rg52\/ref52","unstructured":"Reinchenbach, H., and Dworkin, M. 1981. The orderCytophagales(with addenda on the generaHerpetosiphon,SaprospiraandFlexithrix).InThe prokaryotes.Edited byM.P. Starr, H. Stolp, H.G. Tr\u00fcper, A. Balows, and H.G. Schlegel. Springer-Verlag, Berlin. pp.\u00a0356\u2013379."},{"key":"rg53\/ref53","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.2.578-587.2000"},{"key":"rg54\/ref54","unstructured":"Sato, S., and Shimizu, Y. 1998. Purification of a fluorescent product from the bacterium,Moraxella: a neosaxitoxin impostor.InHarmful algae.Edited byB. Reguera, J. Blanco, M.L. Fern\u00e1ndez, and T. Wyatt. Xunta de Galicia and IOC of UNESCO, Vigo, Spain. pp.\u00a0465\u2013467."},{"key":"rg55\/ref55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-7345.1990.tb00529.x"},{"key":"rg56\/ref56","doi-asserted-by":"publisher","DOI":"10.1080\/10641269309388538"},{"key":"rg57\/ref57","first-page":"643","volume":"7","author":"Shumway S.E.","year":"1988","journal-title":"J. Shellfish Res."},{"key":"rg58\/ref58","doi-asserted-by":"publisher","DOI":"10.1007\/BF02545855"},{"key":"rg59\/ref59","first-page":"1","volume":"26","author":"Silva E.S.","year":"1962","journal-title":"Notas Estud. Inst. Biol. Mar\u00edt."},{"key":"rg60\/ref60","unstructured":"Smayda, T.J. 1990. Novel and nuisance phytoplankton blooms in the sea: evidence for a global epidemic.Edited byE. Gran\u00e9li, B. Sandstr\u00f6m, L. Edler, and D.M. Anderson. Elsevier Science, New York. pp.\u00a029\u201340."},{"key":"rg61\/ref61","unstructured":"Smibert, R.M., and Krieg, N.R. 1981. General characterization.InManual of methods for general bacteriology. Vol.\u00a020.Edited byP. Gerhardt, R.G.E. Murray, R.N. Cotilow, E.W. Nester, W.A. Wood, N.R. Krieg, and G.B. Phillips. American Society for Microbiology, Washington, D.C. pp.\u00a0409\u2013443."},{"key":"rg62\/ref62","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1099\/00221287-72-2-377","volume":"72","author":"Sneath P.H.A.","year":"1972","journal-title":"J. Gen. Microbiol."},{"key":"rg63\/ref63","doi-asserted-by":"publisher","DOI":"10.1017\/S0967026201003262"},{"key":"rg64\/ref64","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1128\/AEM.33.4.940-946.1977","volume":"33","author":"Watson S.W.","year":"1977","journal-title":"Appl. Environ. Microbiol."},{"key":"rg65\/ref65","unstructured":"West, P., and Colwell, R.R. 1984. Identification and classification ofVibrionaceae: an overview.InVibrios in the environment.Edited byR.R. Colwell. John Wiley & Sons, New York. pp.\u00a0285\u2013363."},{"key":"rg66\/ref66","first-page":"239","volume":"5","author":"Zobell C.E.","year":"1944","journal-title":"Bull. Scripps Inst. Oceanogr."}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W09-047","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W09-047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T11:06:58Z","timestamp":1590232018000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W09-047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":65,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1139\/W09-047"],"URL":"https:\/\/doi.org\/10.1139\/w09-047","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]}}}