{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:43:21Z","timestamp":1760888601740},"reference-count":16,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p> Staphylococcus epidermidis biofilms with different proportions of viable but nonculturable bacteria were used to show that SYBR green (SYBR) may be used as a probe to evaluate the bacterial physiological state using flow cytometry. Biofilms grown in excess glucose presented significantly higher proportions of dormant bacteria than biofilms grown in excess glucose with buffered pH conditions or with exponential-phase planktonic cultures. Bacteria obtained from biofilms with high or low proportions of viable but nonculturable cells were further cultured in broth medium and stained with SYBR at different time points. An association between bacterial growth and SYBR staining intensity was observed. In addition, bacteria presenting higher SYBR fluorescence intensity also stained more intensely with cyanoditolyl tetrazolium chloride, used as a probe to evaluate cellular metabolism. Accordingly, planktonic bacteria treated with rifampicin, an inhibitor of bacterial RNA transcription, presented lower SYBR and cyanoditolyl tetrazolium chloride staining intensity than nontreated bacteria. Overall, our results indicate that SYBR, in addition to being used as a component of LIVE\/DEAD stain, may also be used as a probe to evaluate the physiological state of S.\u00a0epidermidis cells. <\/jats:p>","DOI":"10.1139\/w11-078","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T21:43:03Z","timestamp":1317332583000},"page":"850-856","source":"Crossref","is-referenced-by-count":49,"title":["SYBR green as a fluorescent probe to evaluate the biofilm physiological state of <i>Staphylococcus epidermidis<\/i>, using flow cytometry"],"prefix":"10.1139","volume":"57","author":[{"given":"Filipe","family":"Cerca","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunologia, Departamento de Imunofisiologia e Farmacologia (IMFF), Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar\u00a0\u2013 Universidade do Porto (ICBAS\u2013UP), Largo do Professor Abel Salazar 2, 4099-003, Porto, Portugal."},{"name":"Instituto de Biologia Molecular e Celular (IBMC), Rua do Campo Alegre 83, Porto, Portugal."}]},{"given":"Gabriela","family":"Trigo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia, Departamento de Imunofisiologia e Farmacologia (IMFF), Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar\u00a0\u2013 Universidade do Porto (ICBAS\u2013UP), Largo do Professor Abel Salazar 2, 4099-003, Porto, Portugal."},{"name":"Instituto de Biologia Molecular e Celular (IBMC), Rua do Campo Alegre 83, Porto, Portugal."}]},{"given":"Alexandra","family":"Correia","sequence":"additional","affiliation":[{"name":"Centro de Biologia Molecular e Ambiental (CMBA), Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal."}]},{"given":"Nuno","family":"Cerca","sequence":"additional","affiliation":[{"name":"Centro de Engenharia Biol\u00f3gica\u00a0\u2013 Instituto de Biotecnologia e Bioengenharia (CEB\u2013IBB), Campus de Gualtar, Universidade do Minho, Braga, Portugal."}]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[{"name":"Centro de Engenharia Biol\u00f3gica\u00a0\u2013 Instituto de Biotecnologia e Bioengenharia (CEB\u2013IBB), Campus de Gualtar, Universidade do Minho, Braga, Portugal."}]},{"given":"Manuel","family":"Vilanova","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia, Departamento de Imunofisiologia e Farmacologia (IMFF), Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar\u00a0\u2013 Universidade do Porto (ICBAS\u2013UP), Largo do Professor Abel Salazar 2, 4099-003, Porto, Portugal."},{"name":"Instituto de Biologia Molecular e Celular (IBMC), Rua do Campo Alegre 83, Porto, Portugal."}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO;2-M"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02750-06"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/22.6.777"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01601.x"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/016576-0"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"issue":"10","key":"refg7\/ref7","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.1128\/AEM.59.10.3187-3196.1993","volume":"59","author":"Kaprelyants A.S.","year":"1993","journal-title":"Appl. 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