{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T04:25:19Z","timestamp":1754195119066},"reference-count":42,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p> Insulin sensitivity is maximal in the postprandial state, decreasing with a fasting period through a mechanism that is dependent on the integrity of the hepatic parasympathetic nerves\/nitric oxide (NO) production and increased hepatic glutathione (GSH) levels. GSH and NO react to form S-nitrosoglutathione (GSNO), an S-nitrosothiol (RSNO) for which the in-vivo effects are still being determined. The goal of this study was to test the hypothesis that in-vivo administration of RSNOs, GSNO, or S-nitroso-N-acetylpenicillamine (SNAP) increases insulin sensitivity in fasted or fed-denervated animals, but not in fed animals, where full postprandial insulin sensitivity is achieved. Fasted, fed, or fed-denervated male Wistar rats were used as models for different insulin sensitivity conditions. The rapid insulin sensitivity test (RIST) was used to measure insulin-stimulated glucose disposal before and after drug administration (GSNO, SNAP, or 3-morpholinosydnonimine (SIN-1), intravenous (i.v.) or to the portal vein (i.p.v.)). Fast insulin sensitivity was not altered by administration of SIN-1 (neither i.v. nor i.p.v.). Intravenous infusion of RSNOs in fasted and fed hepatic denervated rats increased insulin sensitivity by 126.35%\u00a0\u00b1 35.43% and 82.7%\u00a0\u00b1 12.8%, respectively. In fed animals, RSNOs decreased insulin sensitivity indicating a negative feedback mechanism. These results suggest that RSNOs incremental effect on insulin sensitivity represent a promising therapeutical tool in insulin resistance states. <\/jats:p>","DOI":"10.1139\/y2012-090","type":"journal-article","created":{"date-parts":[[2012,7,5]],"date-time":"2012-07-05T16:04:07Z","timestamp":1341504247000},"page":"887-894","source":"Crossref","is-referenced-by-count":6,"title":["Understanding the in-vivo relevance of <i>S<\/i>-nitrosothiols in insulin action"],"prefix":"10.1139","volume":"90","author":[{"given":"Ana B.","family":"Fernandes","sequence":"first","affiliation":[{"name":"CEDOC, Universidade Nova de Lisboa, Campo M\u00e1rtires da P\u00e1tria 130, 1169-056 Lisboa, Portugal."}]},{"given":"Maria P.","family":"Guarino","sequence":"additional","affiliation":[{"name":"CEDOC, Universidade Nova de Lisboa, Campo M\u00e1rtires da P\u00e1tria 130, 1169-056 Lisboa, Portugal."}]},{"given":"M. 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