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The patterns can be induced only inside a very narrow time window, ~ 5 min. Cells attachment then develops, and pattern formation can no longer be induced. The time window for pattern formation was diminished by favoring cell attachment, for instance by treating culture dishes with cationic macromolecules, such as poly-L-Lysine. It was increased by cooling the cells to 18\u00b0C, or by a prolonged exposure of the cells to trypsin, which is known to digest adhesion molecules.<\/jats:p><jats:p>The Faraday instability leads to a method to characterize cell attachment. It also permits the production of heterogeneous cultures with several cell types, with a well controlled heterogeneity. This can be used to study heterotypic cell interactions in vitro.<\/jats:p>","DOI":"10.1142\/s0218127402005662","type":"journal-article","created":{"date-parts":[[2002,10,3]],"date-time":"2002-10-03T12:38:06Z","timestamp":1033648686000},"page":"2009-2019","source":"Crossref","is-referenced-by-count":5,"title":["THE USE OF FARADAY INSTABILITY TO PRODUCE DEFINED TOPOLOGICAL ORGANIZATION IN CULTURES OF MAMMALIAN CELLS"],"prefix":"10.1142","volume":"12","author":[{"given":"SEIJI","family":"TAKAGI","sequence":"first","affiliation":[{"name":"Institut Non Lin\u00e9aire de Nice, 1361, Route des Lucioles 06560 Valbonne, France"}]},{"given":"VALENTIN","family":"KRINSKY","sequence":"additional","affiliation":[{"name":"Institut Non Lin\u00e9aire de Nice, 1361, Route des Lucioles 06560 Valbonne, France"}]},{"given":"ALAIN","family":"PUMIR","sequence":"additional","affiliation":[{"name":"Institut Non Lin\u00e9aire de Nice, 1361, Route des Lucioles 06560 Valbonne, France"}]},{"given":"CHRISTIAN","family":"FRELIN","sequence":"additional","affiliation":[{"name":"Institute de Pharmacologie Mol\u00e9culaire et Cellulaire, 660, Route des Lucioles, 06560 Valbonne, France"}]}],"member":"219","published-online":{"date-parts":[[2011,11,20]]},"reference":[{"key":"rf1","volume-title":"An Introduction to Fluid Dynamics","author":"Batchelor G. 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