{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:01:55Z","timestamp":1761512515479},"reference-count":18,"publisher":"World Scientific Pub Co Pte Lt","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bioinform. Comput. Biol."],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p> Determining glycan structures is vital to comprehend cell-matrix, cell-cell, and even intracellular biological events. Glycan sequencing, which determines the primary structure of a glycan using tandem mass spectrometry (MS\/MS), remains one of the most important tasks in proteomics. Analogous to peptide de novo sequencing, glycan de novo sequencing determines the structure without the aid of a known glycan database. We show in this paper that glycan de novo sequencing is NP-hard. We then provide a heuristic algorithm and develop a software program to solve the problem in practical cases. Experiments on real MS\/MS data of glycopeptides demonstrate that our heuristic algorithm gives satisfactory results on practical data. <\/jats:p>","DOI":"10.1142\/s0219720008003291","type":"journal-article","created":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T10:43:22Z","timestamp":1204800202000},"page":"77-91","source":"Crossref","is-referenced-by-count":23,"title":["COMPLEXITIES AND ALGORITHMS FOR GLYCAN SEQUENCING USING TANDEM MASS SPECTROMETRY"],"prefix":"10.1142","volume":"06","author":[{"given":"BAOZHEN","family":"SHAN","sequence":"first","affiliation":[{"name":"Department of Computer Science, University of Western Ontario, London, Ontario, N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BIN","family":"MA","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of Western Ontario, London, Ontario, N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KAIZHONG","family":"ZHANG","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of Western Ontario, London, Ontario, N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GILLES","family":"LAJOIE","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Western Ontario, London, Ontario, N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"219","published-online":{"date-parts":[[2011,11,21]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01511"},{"key":"rf2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058890"},{"key":"rf3","doi-asserted-by":"publisher","DOI":"10.1007\/BF01049915"},{"key":"rf4","first-page":"161","volume":"23","author":"Zala J.","journal-title":"Mass Spectrom. 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