{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T23:07:42Z","timestamp":1776899262851,"version":"3.51.2"},"reference-count":19,"publisher":"World Scientific Pub Co Pte Lt","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bioinform. Comput. Biol."],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p> A common method for calculating results from qPCR experiments is the comparative Ct method, also called the 2<jats:sup>-\u0394\u0394Ct<\/jats:sup> method. However, several assumptions are included in the 2<jats:sup>-\u0394\u0394Ct<\/jats:sup> method and standard statistical analyses are not directly applicable. Here, we describe a different method, the X<jats:sub>0<\/jats:sub> method, for result calculations and statistical analysis from qPCR experiments. The X<jats:sub>0<\/jats:sub> method differs from the 2<jats:sup>-\u0394\u0394Ct<\/jats:sup> method by introducing a conversion of the exponentially related Ct values into linearly related X<jats:sub>0<\/jats:sub> values, which represent the amount of starting material in a qPCR experiment. Results calculated by the X<jats:sub>0<\/jats:sub> method are illustrated for qPCR experiments with technical and biological replicates, including procedures to calculate standard deviations. Incorporation of primer efficiencies in calculations by the X<jats:sub>0<\/jats:sub> method is also described. Altogether, the X<jats:sub>0<\/jats:sub> method constitutes a very simple and accurate alternative to the 2<jats:sup>-\u0394\u0394Ct<\/jats:sup> method for result calculations from qPCR data. <\/jats:p>","DOI":"10.1142\/s0219720010004963","type":"journal-article","created":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T06:24:05Z","timestamp":1277101445000},"page":"885-900","source":"Crossref","is-referenced-by-count":106,"title":["ANALYSIS OF qPCR DATA BY CONVERTING EXPONENTIALLY RELATED Ct VALUES INTO LINEARLY RELATED X<sub>0<\/sub> VALUES"],"prefix":"10.1142","volume":"08","author":[{"given":"RUNE","family":"THOMSEN","sequence":"first","affiliation":[{"name":"Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"given":"CHRISTINA ANE ELISABETH","family":"S\u00d8LVSTEN","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"given":"TOKE ELBEK","family":"LINNET","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"given":"JENNY","family":"BLECHINGBERG","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"given":"ANDERS LADE","family":"NIELSEN","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmark"}]}],"member":"219","published-online":{"date-parts":[[2011,11,21]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"rf2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.995"},{"key":"rf3","first-page":"155","volume":"15","author":"Bustin S. A.","journal-title":"J. Biomol. Tech."},{"key":"rf4","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-006-0097-6"},{"key":"rf5","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"rf6","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"rf7","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"rf8","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"rf9","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-120"},{"key":"rf10","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-2-r19"},{"key":"rf11","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern305"},{"key":"rf12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2008.00346.x"},{"key":"rf13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006162"},{"key":"rf14","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"rf15","volume":"3","author":"Vandesompele J.","journal-title":"Genome. Biol."},{"key":"rf16","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng122"},{"key":"rf17","doi-asserted-by":"publisher","DOI":"10.1080\/09553000210158056"},{"key":"rf18","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)01423-4"},{"key":"rf19","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-85"}],"container-title":["Journal of Bioinformatics and Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.worldscientific.com\/doi\/pdf\/10.1142\/S0219720010004963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T13:11:08Z","timestamp":1565183468000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.worldscientific.com\/doi\/abs\/10.1142\/S0219720010004963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":19,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2011,11,21]]},"published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1142\/S0219720010004963"],"URL":"https:\/\/doi.org\/10.1142\/s0219720010004963","relation":{},"ISSN":["0219-7200","1757-6334"],"issn-type":[{"value":"0219-7200","type":"print"},{"value":"1757-6334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10]]}}}