{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T05:29:24Z","timestamp":1649827764066},"reference-count":22,"publisher":"World Scientific Pub Co Pte Lt","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bioinform. Comput. Biol."],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p> A common post-transcriptional modification of RNA is the conversion of uridine to its isomer pseudouridine. We investigated the biological significance of eukaryotic pseudouridine synthases using the yeast Saccharomyces cerevisiae. We conducted a comprehensive statistical analysis on growth data from automated perturbation (gene deletion) experiments, and used bi-logistic curve analysis to characterise the yeast phenotypes. The deletant strains displayed different alteration in growth properties, including in some cases enhanced growth and\/or biphasic growth curves not seen in wild-type strains under matched conditions. These results demonstrate that disrupting pseudouridine synthases can have a significant qualitative effect on growth. We further investigated the significance of post-transcriptional pseudouridine modification through investigation of the scientific literature. We found that (1) In Toxoplasma gondii, a pseudouridine synthase gene is critical in cellular differentiation between the two asexual forms: Tachyzoites and bradyzoites; (2) Mutation of pseudouridine synthase genes has also been implicated in human diseases (mitochondrial myopathy and sideroblastic anemia (MLASA); dyskeratosis congenita). Taken together, these results are consistent with pseudouridine synthases having a Gene Ontology function of \"biological regulation\". <\/jats:p>","DOI":"10.1142\/s0219720014500152","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T04:07:55Z","timestamp":1403064475000},"page":"1450015","source":"Crossref","is-referenced-by-count":1,"title":["An investigation into eukaryotic pseudouridine synthases"],"prefix":"10.1142","volume":"12","author":[{"given":"Ross D.","family":"King","sequence":"first","affiliation":[{"name":"Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Aberystwyth University, Aberystwyth, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"219","published-online":{"date-parts":[[2014,8,25]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.03.085"},{"key":"rf2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(02)01402-5"},{"key":"rf3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1029"},{"key":"rf4","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr948"},{"key":"rf5","doi-asserted-by":"publisher","DOI":"10.1261\/rna.100806"},{"key":"rf6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409581200"},{"key":"rf7","doi-asserted-by":"publisher","DOI":"10.1261\/rna.7770604"},{"key":"rf8","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165620"},{"key":"rf9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jala.2009.10.001"},{"key":"rf10","volume-title":"An R Companion to Applied Regression","author":"Fox J.","year":"2011"},{"key":"rf11","doi-asserted-by":"publisher","DOI":"10.1016\/0040-1625(94)90042-6"},{"key":"rf12","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5338.680"},{"key":"rf13","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-11-0968"},{"key":"rf14","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.316"},{"key":"rf15","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00329-08"},{"key":"rf16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500216200"},{"key":"rf17","doi-asserted-by":"publisher","DOI":"10.2174\/1566524053586581"},{"key":"rf18","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.09.017"},{"key":"rf19","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0414"},{"key":"rf20","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1743009"},{"key":"rf21","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.11.7461"},{"key":"rf22","first-page":"25","volume":"25","year":"2000","journal-title":"Gene. 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