{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:29:21Z","timestamp":1760016561145},"reference-count":11,"publisher":"World Scientific Pub Co Pte Lt","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p> Radix Adenophorae (Shashen), a traditional Chinese medicine commonly used as an antitussive and expectorant, is derived from roots of Adenophora stricta Miq. and Adenophora tetraphylla (Thunb.) Fisch. Twelve species and varieties of Adenophora and Glehnia, however, could act as substitutes or adulterants of Radix Adenophorae on the commercial markets in South East Asia, and roots of Adenophora hunanensis Nannf. and Glihnia littoralis F. Schmidt ex Miq. are the most common examples. The authentic identification of dried roots of A. stricta and A. tetraphylla, however, is difficult on the basis of appearance and morphology. A molecular genetic approach was developed here to identify the species of Radix Adenophorae. The 5S-rRNA spacer domains (~250 bp) were amplified by the polymerase chain reaction (PCR) from genomic DNAs isolated from A. stricta, A. tetraphylla, A. hunanensis and G. littoralis, and subsequently, the nucleotide sequences were determined. Diversity in DNA sequence and restriction enzyme mapping among various species were found in their 5S-rRNA spacer domains, which could serve as markers for authentic identification of Radix Adenophorae. <\/jats:p>","DOI":"10.1142\/s0192415x03001612","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T10:41:20Z","timestamp":1076323280000},"page":"919-926","source":"Crossref","is-referenced-by-count":12,"title":["Genetic Distinction of Radix Adenophorae From Its Adulterants by the  DNA Sequence of 5S-rRNA Spacer Domains"],"prefix":"10.1142","volume":"31","author":[{"given":"K. J.","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Biology and Biotechnology  Research Institute, The Hong Kong University of Science and Technology,  Hong Kong, China"},{"name":"Department of Pharmacy, Beijing Military Medical College  of PLA, Beijing, 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. T. X.","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology  Research Institute, The Hong Kong University of Science and Technology,  Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. M.","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology  Research Institute, The Hong Kong University of Science and Technology,  Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. F.","family":"Tu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Beijing University, Beijing,  100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. W. K.","family":"Tsim","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology  Research Institute, The Hong Kong University of Science and Technology,  Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"219","published-online":{"date-parts":[[2012,4,5]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1055\/s-1999-14003"},{"key":"rf2","unstructured":"S. J.\u00a0Gan, Z. J.\u00a0Li and J. Q.\u00a0Zhou, The Development Strategy on TCM Modernization (Scientific and Technological Literature Press, Beijing, 1998)\u00a0p. 126."},{"key":"rf3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.21.4851"},{"key":"rf4","first-page":"726","volume":"49","author":"Long E. O.","journal-title":"Ann. Rev. Biochem."},{"key":"rf5","unstructured":"J. P.\u00a0Luo, Pharmacognosy (Chinese Scientific and Technological Press of Medicine, Beijing, 1996)\u00a0p. 170."},{"key":"rf6","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)00111-4"},{"key":"rf7","doi-asserted-by":"publisher","DOI":"10.1021\/jf0202279"},{"key":"rf8","unstructured":"J.\u00a0Sambrook and D. W.\u00a0Russell, Molecular Cloning: A Laboratory Manual, 3rd edn.\u00a01 (Cold Spring Harbor Laboratory Press, New York, 1989)\u00a0pp. 1.32\u20131.34."},{"key":"rf9","unstructured":"G. J.\u00a0Xu and L. S.\u00a0Xu, Species Systematization and Quality Evaluation of Commonly used Chinese Traditional Drugs\u00a0II (Science and Technology Press of Fujian, Fuzhou, 1997)\u00a0pp. 1\u201354."},{"key":"rf10","doi-asserted-by":"publisher","DOI":"10.1021\/jf026178h"},{"key":"rf11","unstructured":"X. Y.\u00a0Zheng, Pharmacopoeia of the People's Republic of China (Chemical Industry Press, Beijing, 2000)\u00a0p. 75, 197."}],"container-title":["The American Journal of Chinese Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.worldscientific.com\/doi\/pdf\/10.1142\/S0192415X03001612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T00:51:24Z","timestamp":1565139084000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.worldscientific.com\/doi\/abs\/10.1142\/S0192415X03001612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":11,"journal-issue":{"issue":"06","published-online":{"date-parts":[[2012,4,5]]},"published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1142\/S0192415X03001612"],"URL":"https:\/\/doi.org\/10.1142\/s0192415x03001612","relation":{},"ISSN":["0192-415X","1793-6853"],"issn-type":[{"value":"0192-415X","type":"print"},{"value":"1793-6853","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}