{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:42:37Z","timestamp":1780504957917,"version":"3.54.1"},"reference-count":42,"publisher":"World Scientific Pub Co Pte Lt","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p> Hepatic stellate cells (HSCs) undergo activation during the development of liver fibrosis. Transcription factor myocyte enhancer factor (MEF2) 2C plays a key role in this process. In the present study, we investigated the effect of hydroxysafflor yellow A (HSYA) on hepatic fibrosis and further investigated potential mechanisms in vivo. Sprague-Dawley rats were administered with CCl<jats:sub>4<\/jats:sub> together with or without HYSA for 12 weeks. The effect of HYSA on hepatic fibrosis was evaluated using hematoxylin-eosin and Van Gieson staining. Messenger RNA expression was quantified by real-time polymerase chain reaction, and protein was quantified by Western blot or immunohistochemistry. Our results revealed that CCl<jats:sub>4<\/jats:sub> treatment induced micronodular hepatic fibrosis with a pronounced deposition of collagen fibers. Treatment with HYSA resulted in a significant decrease in fibrosis, protein expression of \u03b1-SMA, and MEF-2C gene expression. This was accompanied by a decreased expression of T\u03b2-RI, T\u03b2-RII, MEKK3, MEK5, and phosphorylation of ERk5. HYSA alone had no effect on the measured parameters. Our findings demonstrate that HSYA protected, at least in part, the rat liver from CCl <jats:sub>4<\/jats:sub>-caused fibrogenesis through inhibition of hepatic stellate cell (HSC) activation, attenuation of transforming growth factor beta (TGF-\u03b2) signaling. HSYA may become a novel and promising agent for the inhibition of hepatic fibrosis. <\/jats:p>","DOI":"10.1142\/s0192415x12500371","type":"journal-article","created":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T07:21:13Z","timestamp":1337066473000},"page":"481-494","source":"Crossref","is-referenced-by-count":20,"title":["Hydroxysafflor Yellow A Attenuates Carbon Tetrachloride-Induced Hepatic Fibrosis in Rats by Inhibiting Erk5 Signaling"],"prefix":"10.1142","volume":"40","author":[{"given":"Ying-Bo","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Ying","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zou","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Cheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Cai","family":"Niu","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"219","published-online":{"date-parts":[[2012,5,16]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gastro.2003.07.011"},{"key":"rf2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2010.03.012"},{"key":"rf3","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1117"},{"key":"rf4","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(03)00216-2"},{"key":"rf5","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24169"},{"key":"rf6","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.04.031"},{"key":"rf7","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2010.07.006"},{"key":"rf8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.21.022404.142018"},{"key":"rf9","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2010.010231"},{"key":"rf10","first-page":"1266","volume":"13","author":"Ikeda K.","journal-title":"J. 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