{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:30:36Z","timestamp":1783002636740,"version":"3.54.5"},"reference-count":24,"publisher":"World Scientific Pub Co Pte Lt","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p> Endothelin-1 (ET-1) is a powerful vasoconstrictor that contributes to blood pressure elevation. The biological effects of ETs are mediated by two receptors, namely, endothelin type A receptor (ET<jats:sub>A<\/jats:sub>R) and endothelin type B receptor (ET<jats:sub>B<\/jats:sub>R). Chinese herbal medicines (CHM) with antagonist activity for these two receptors were screened by establishing stable clones of CHO-K1 cells expressing high levels of human ET<jats:sub>A<\/jats:sub>R and ET<jats:sub>B<\/jats:sub>R, namely CHO-ET<jats:sub>A<\/jats:sub>R and CHO-ET<jats:sub>B<\/jats:sub>R.The aqueous extract of Prunellae Spica (P1) inhibited the binding of <jats:sup>125<\/jats:sup>I-ET-1 to ET<jats:sub>A<\/jats:sub>R and ET<jats:sub>B<\/jats:sub>R in CHO-ET<jats:sub>A<\/jats:sub>R and CHO-ET<jats:sub>B<\/jats:sub>R cells, respectively. P1 suppressed the ET-1-induced mobilization of intracellular Ca<jats:sup>2+<\/jats:sup> . Through the alcohol fractionation of P1, the antagonists of human ET<jats:sub>A<\/jats:sub>R and ET<jats:sub>B<\/jats:sub>R were found to belong to different, separable ingredients and the antagonist of ET<jats:sub>A<\/jats:sub>R is more soluble in alcohol. The two antagonists were also effective in the test on human primary cells, HASMC and HUVEC. P1 successfully prevented the development of ET-1-associated hypertension in rats without further purification. These results indicate the presence of anti-hypertensive ingredients in P. Spica extract, at least through the inactivation of ET<jats:sub>A<\/jats:sub>R and\/or ET<jats:sub>B<\/jats:sub>R. <\/jats:p>","DOI":"10.1142\/s0192415x13500079","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T06:29:09Z","timestamp":1358836149000},"page":"85-98","source":"Crossref","is-referenced-by-count":6,"title":["<i>Prunellae Spica<\/i> Extract Contains Antagonists for Human Endothelin Receptors"],"prefix":"10.1142","volume":"41","author":[{"given":"Chih-Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C."},{"name":"Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei 112, Taiwan, R.O.C."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Low-Tone","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C."},{"name":"Institute of Physiology, National Yang-Ming University, Taipei 112, Taiwan, R.O.C."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Yuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Chang","family":"Juan","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C."},{"name":"Institute of Physiology, National Yang-Ming University, Taipei 112, Taiwan, R.O.C."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo-Chun","family":"Au","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C."},{"name":"Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei 112, Taiwan, R.O.C."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"219","published-online":{"date-parts":[[2013,1,21]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1038\/348730a0"},{"key":"rf2","first-page":"600","volume":"68","author":"Battistini B.","year":"1993","journal-title":"Lab. 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