{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:52:10Z","timestamp":1792615930991,"version":"4.5.0"},"reference-count":32,"publisher":"World Scientific Pub Co Pte Ltd","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>For centuries, natural plant extracts have played an important role in traditional medicine for curing and preventing diseases. Studies have revealed that Artocarpus communis possess various bioactivities, such as anti-inflammation, anti-oxidant, and anticancer activities. A. communis offers economic value as a source of edible fruit, yields timber, and is widely used in folk medicines. However, little is known about its molecular mechanisms of anticancer activity. Here, we demonstrate the antiproliferative activity of A. communis methanol extract (AM) and its dichloromethane fraction (AD) in two human hepatocellular carcinoma (HCC) cell lines, HepG2 and PLC\/PRF\/5. Colony assay showed the long-term inhibitory effect of both extracts on cell growth. DNA laddering and immunoblotting analyses revealed that both extracts did not induce apoptosis in the hepatoma cell lines. AM and AD-treated cells demonstrated different cell cycle distribution compared to UV-treated cells, which presented apoptotic cell death with high sub-G1 ratio. Instead, acridine orange staining revealed that AM and AD triggered autophagosome accumulation. Immunoblotting showed a significant expression of autophagy-related proteins, which indicated the autophagic cell death (ACD) of hepatoma cell lines. This study therefore demonstrates that A. communis AM and its dichloromethane fraction can induce ACD in HCC cells and elucidates the potential of A. communis extracts for development as anti tumor therapeutic agents that utilize autophagy as mechanism in mediating cancer cell death.<\/jats:p>","DOI":"10.1142\/s0192415x15500354","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T08:47:12Z","timestamp":1431506832000},"page":"559-579","source":"Crossref","is-referenced-by-count":15,"title":["<i>Artocarpus communis<\/i>Induces Autophagic Instead of Apoptotic Cell Death in Human Hepatocellular Carcinoma Cells"],"prefix":"10.1142","volume":"43","author":[{"given":"Cheng-Wei","family":"Tzeng","sequence":"first","affiliation":[{"name":"Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Sheng","family":"Tzeng","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Chi Mei Medical Center, Tainan, Taiwan"},{"name":"Department of Medical Imaging and Radiological Science, College of Health Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Tzung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiang-Wen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nursing, Division of Basic Medical Sciences and Chronic Diseases and Health Promotion Research Center, Chang Gung Institute of Technology, Chia-Yi, Taiwan"},{"name":"Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Hong","family":"Yen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Lin","family":"Yen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan"},{"name":"Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan"},{"name":"Institute of Biomedical Sciences, Sun Yat-Sen University, Kaohsiung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Ching","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan"},{"name":"School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"219","published-online":{"date-parts":[[2015,5,13]]},"reference":[{"key":"rf1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-010-0425-6"},{"key":"rf2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2009.08.001"},{"key":"rf3","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1666"},{"key":"rf5","doi-asserted-by":"publisher","DOI":"10.1021\/jf8017436"},{"key":"rf6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61347-0"},{"key":"rf7","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.339"},{"key":"rf8","doi-asserted-by":"crossref","first-page":"2450","DOI":"10.1096\/fasebj.6.7.1563596","volume":"6","author":"Gerschenson L. 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