{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T03:41:33Z","timestamp":1780544493705,"version":"3.54.1"},"reference-count":26,"publisher":"World Scientific Pub Co Pte Lt","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p> Punicalagin (PUN), a major bioactive component in pomegranate juice, has been proven to exert neuroprotective effects against cerebral ischemia\/reperfusion (I\/R) insult via anti-oxidant properties. This study aims to investigate whether PUN provides cardioprotection against myocardial I\/R (MI\/R) injury and the underlying mechanisms. PUN (30[Formula: see text]mg\/kg\/d) or vehicle was intragastrically administered to Sprague-Dawley rats for one week before the operation. MI\/R was induced by ligating the left anterior descending coronary artery for 30[Formula: see text]min and subsequent reperfusion for 3[Formula: see text]h. PUN pretreatment conferred cardioprotective effects against MI\/R injury by improving cardiac function, limiting infarct size, reducing serum creatine kinase-MB and lactate dehydrogenase activities, and suppressing cardiomyocyte apoptosis. Moreover, PUN pretreatment inhibited I\/R-induced myocardial oxidative stress as evidenced by decreased generation of superoxide content and malonaldialdehyde formation and increased antioxidant capability. Furthermore, PUN pretreatment increased adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation in I\/R hearts. AMPK inhibitor compound c inhibited PUN-enhanced AMPK phosphorylation, and blunted PUN-mediated anti-oxidative effects and cardioprotection. These results indicate for the first time that PUN pretreatment protect against I\/R-induced oxidative stress and myocardial injury via activation of AMPK. <\/jats:p>","DOI":"10.1142\/s0192415x17500057","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T09:28:07Z","timestamp":1484299687000},"page":"53-66","source":"Crossref","is-referenced-by-count":38,"title":["Punicalagin Pretreatment Attenuates Myocardial Ischemia-Reperfusion Injury via Activation of AMPK"],"prefix":"10.1142","volume":"45","author":[{"given":"Mingge","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi\u2019an, P.R. China"},{"name":"Department of Geriatrics, Xi\u2019an Central Hospital, Xi\u2019an, P.R. 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