{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:37:04Z","timestamp":1764603424202},"reference-count":38,"publisher":"World Scientific Pub Co Pte Ltd","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Antrodia cinnamomea (A.\u00a0cinnamomea), a popular medicinal mushroom in Taiwan, is widely used to prevent or treat liver diseases. Systematic studies on the anti-inflammatory effect of A.\u00a0cinnamomea and its molecular mechanisms have not yet been fully investigated. HPLC fingerprint analysis identified seven ergostane-type triterpenoids from A.\u00a0cinnamomea water extract (ACW), including high amounts of Antcin K (AC), Antcin C, Antcin H, Dehydrosulphurenic acid, Antcin B, Antcin A and Dehydroeburicoic acid. Here, we explored the effects and mechanisms of ACW and the highest content AC on N-nitrosodiethylamine (DEN) induced liver inflammation, fibrosis and carcinogenesis in rats. In the in vitro study, we measured how ACW and AC dose-dependently scavenged O[Formula: see text], H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>and HOCl by a chemiluminescence analyzer. In the in vivo experiment, oral intake ACW and AC significantly inhibited DEN-enhanced hepatocellular inflammation, fibrosis and carcinoma by pathologic observation, the elevated bile and liver reactive oxygen species (ROS) amounts, plasma [Formula: see text]-glutamyl transpeptidase, and oxidative stress including 3-nitrotyrosine, 4-hydroxynonenal and Kuppfer cell infiltration (ED-1 stains) in the inflammatory livers. DEN enhanced nuclear factor-[Formula: see text]B (NF-[Formula: see text]B) translocation, whereas ACW and AC suppressed DEN-enhanced NF-[Formula: see text]B translocation through the inhibition of its upstream signaling of p85\/phosphoinositide-3-kinase, mitogen activated protein kinase and CYP2E1 expression. In conclusion, DEN can induce hepatocellular inflammation, fibrosis and carcinoma by increasing NF-[Formula: see text]B translocation to the nucleus, and oxidative injury. ACW and its active component, Antcin K, counteract DEN-induced hepatic injury and inflammation by the protective and therapeutic mechanisms of a direct scavenging ROS activity and an upregulation of anti-oxidant defense mechanisms.<\/jats:p>","DOI":"10.1142\/s0192415x17500124","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T09:28:07Z","timestamp":1484299687000},"page":"173-198","source":"Crossref","is-referenced-by-count":26,"title":["Fruiting Bodies of<i>Antrodia cinnamomea<\/i>and Its Active Triterpenoid, Antcin K, Ameliorates N-Nitrosodiethylamine-Induced Hepatic Inflammation, Fibrosis and Carcinogenesis in Rats"],"prefix":"10.1142","volume":"45","author":[{"given":"An-Jan","family":"Tien","sequence":"first","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-Yen","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Surgery, Mackay Memorial Hospital and Mackay Medical College, Taipei 10449, Taiwan, R.O.C"},{"name":"Mackay Junior College of Medicine, Nursing and Management, New Taipei City 252, Taiwan, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueh-Hsi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lung-Chin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kuang-Tien General Hospital, Taichung City 433, Taiwan, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiang-Ting","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"219","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"S0192415X17500124BIB001","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/20.7.1337"},{"key":"S0192415X17500124BIB002","first-page":"3028","volume":"55","author":"Boitier E.","year":"1995","journal-title":"Cancer Res."},{"key":"S0192415X17500124BIB003","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23133"},{"key":"S0192415X17500124BIB004","first-page":"347","volume":"45","author":"Chang T.T.","year":"2004","journal-title":"Botanic Bull. 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