{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T16:27:24Z","timestamp":1772036844335,"version":"3.50.1"},"reference-count":45,"publisher":"World Scientific Pub Co Pte Lt","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p> Antrodia cinnamomea, a medicinal mushroom, has previously demonstrated anti-inflammatory activity, although the specific compound responsible for the effect remains unclear. The present study was designed to investigate the anti-inflammatory property of antrolone, a novel benzoid derived from A. cinnamomea mycelium, and to clarify the underlying mechanisms of action. To this end, murine macrophage RAW264.7 cells were treated with antrolone (0.1\u201330[Formula: see text][Formula: see text]M) 30[Formula: see text]min prior to stimulation with lipopolysaccharides (LPS, 0.1[Formula: see text][Formula: see text]g\/ml) for 24[Formula: see text]h. Cell viability, nitric oxide (NO) and prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) production, levels of pro-inflammatory cytokines and chemokines, and the signaling pathways involved in the inflammatory cascades were then investigated. Our results show that antrolone significantly decreased LPS-induced NO, PGE<jats:sub>2<\/jats:sub>, pro-inflammatory cytokine, and keratinocyte chemoattractant CXCL1 (KC) production and reduced levels of the proteins inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). These effects were independent of the effect of antrolone on macrophage cytotoxicity. Moreover, antrolone significantly inhibited the activation of the NF[Formula: see text]B, MAPK, and AKT pathways, while it increased nuclear factor erythroid-2-related factor (Nrf2) and heme oxygenase-1 (HO-1) levels. Our findings suggest that antrolone exhibits potent anti-inflammatory activity and may, therefore, be a lead compound for the development of an anti-inflammatory drug. <\/jats:p>","DOI":"10.1142\/s0192415x18500684","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T01:45:53Z","timestamp":1535420753000},"page":"1297-1313","source":"Crossref","is-referenced-by-count":21,"title":["Antrolone, a Novel Benzoid Derived from <i>Antrodia cinnamomea<\/i>, Inhibits the LPS-Induced Inflammatory Response in RAW264.7 Macrophage Cells by Balancing the NF-\u03baB and Nrf2 Pathways"],"prefix":"10.1142","volume":"46","author":[{"given":"I-Chuan","family":"Yen","sequence":"first","affiliation":[{"name":"School of Pharmacy, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Shian","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, National Formosa University, Yunlin, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Chieh","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blerina","family":"Ahmetaj-Shala","sequence":"additional","affiliation":[{"name":"National Heart &amp; Lung Institute, Imperial College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsu-Chung","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Yu","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"219","published-online":{"date-parts":[[2018,10,5]]},"reference":[{"key":"S0192415X18500684BIB001","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i4857"},{"key":"S0192415X18500684BIB002","doi-asserted-by":"publisher","DOI":"10.1021\/np070045e"},{"key":"S0192415X18500684BIB003","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301931"},{"key":"S0192415X18500684BIB004","doi-asserted-by":"publisher","DOI":"10.1021\/jf801171x"},{"key":"S0192415X18500684BIB005","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19010022"},{"key":"S0192415X18500684BIB006","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.15.1.8679227"},{"key":"S0192415X18500684BIB007","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep108"},{"key":"S0192415X18500684BIB008","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084800"},{"key":"S0192415X18500684BIB009","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2005.06.013"},{"key":"S0192415X18500684BIB010","doi-asserted-by":"publisher","DOI":"10.1021\/jf903638p"},{"key":"S0192415X18500684BIB011","doi-asserted-by":"publisher","DOI":"10.1021\/jf405113g"},{"key":"S0192415X18500684BIB012","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X17500665"},{"key":"S0192415X18500684BIB013","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.04.053"},{"key":"S0192415X18500684BIB014","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2013.01.014"},{"key":"S0192415X18500684BIB015","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.08.017"},{"key":"S0192415X18500684BIB016","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2012.10.001"},{"key":"S0192415X18500684BIB017","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04019.x"},{"key":"S0192415X18500684BIB018","doi-asserted-by":"publisher","DOI":"10.1038\/nri3452"},{"key":"S0192415X18500684BIB019","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2015.8"},{"key":"S0192415X18500684BIB020","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25383"},{"key":"S0192415X18500684BIB021","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.06.011"},{"key":"S0192415X18500684BIB022","first-page":"718739","volume":"2013","author":"Lee S.Y.","year":"2013","journal-title":"Evid.-Based Complement. 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