{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:27:30Z","timestamp":1768566450159,"version":"3.49.0"},"reference-count":34,"publisher":"World Scientific Pub Co Pte Ltd","issue":"02","funder":[{"DOI":"10.13039\/501100004319","name":"Shin Kong Wu Ho-Su Memorial Hospital","doi-asserted-by":"crossref","award":["SKH-8302-105-DR-23"],"award-info":[{"award-number":["SKH-8302-105-DR-23"]}],"id":[{"id":"10.13039\/501100004319","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p> MicroRNA 145 (miR-145) is a critical modulator of cardiovascular diseases. The downregulation of myocardial miR-145 is followed by an increase in disabled-2 (Dab2) expression in cardiomyocytes. (-)-epigallocatechin gallate (EGCG) is a flavonoid that has been evaluated extensively due to its diverse pharmacological properties including anti-inflammatory effects. The aim of this study was to investigate the cardioprotective effects of EGCG under hypoxia-induced stress in vitro and in vivo. The hypoxic insult led to the suppression of miR-145 expression in cultured rat cardiomyocytes in a concentration-dependent manner. Western blotting and real-time PCR were performed. In rat myocardial infarction study, in situ hybridization, and immunofluorescent analyses were adopted. The western blot and real-time PCR data revealed that hypoxic stress with 2.5% O<jats:sub>2<\/jats:sub> suppressed the expression of miR-145 and Wnt3a\/[Formula: see text]-catenin in cultured rat cardiomyocytes but augmented Dab2. Treatment with EGCG attenuated Dab2 expression, but increased Wnt3a and [Formula: see text]-catenin in hypoxic cultured cardiomyocytes. Following in vivo myocardial infarction (MI) study, the data revealed the myocardial infarct area reduced by 48.5%, 44.6%, and 48.5% in EGCG (50[Formula: see text]mg\/kg) or miR-145 dominant or Dab2 siRNA groups after myocardial infarction for 28 days, respectively. This study demonstrated that EGCG increased miR-145, Wnt3a, and [Formula: see text]-catenin expression but attenuated Dab2 expression. Moreover, EGCG ameliorated myocardial ischemia in vivo. The novel suppressive effect was mediated through the miR-145 and Dab2\/Wnt3a\/[Formula: see text]-catenin pathways. <\/jats:p>","DOI":"10.1142\/s0192415x20500172","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T07:04:33Z","timestamp":1583478273000},"page":"341-356","source":"Crossref","is-referenced-by-count":17,"title":["(-)-Epigallocatechin Gallate Promotes MicroRNA 145 Expression against Myocardial Hypoxic Injury through Dab2\/Wnt3a\/\u03b2-catenin"],"prefix":"10.1142","volume":"48","author":[{"given":"Chiu-Mei","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan"},{"name":"Faculty of Medicine, School of Medicine, Fu Jen Catholic University, Taipei City, Taiwan"},{"name":"Institute of Injury Prevention and Control, College of Public Health, Taipei Medical University, Taipei City, 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