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This study aimed to explore that EGCG ameliorates homocysteine-induced endothelial cell apoptosis through enhancing the sirtuin 1 (SIRT1)\/AMP-activated protein kinase (AMPK) survival signaling pathway. Human umbilical endothelial cells were treated with homocysteine in the presence or absence of EGCG. We found that EGCG significantly increased the activities of SIRT1 and AMPK. EGCG diminished homocysteine-mediated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation by inhibiting protein kinase C activation as well as reactive oxygen species (ROS) generation and recovered the activity of the endogenous antioxidant enzyme, superoxidase dismutase (SOD). Besides, EGCG also restores homocysteine-mediated dephosphorylation of Akt and decreases endothelial NO synthase (eNOS) expression. Furthermore, EGCG ameliorates homocysteine-activated pro-apoptotic events. The present study shows that EGCG prevents homocysteine-induced endothelial cell apoptosis via enhancing SIRT1\/AMPK as well as Akt\/eNOS signaling pathways. Results from this study indicated that EGCG might have some benefits for hyperhomocysteinemia.<\/jats:p>","DOI":"10.1142\/s0192415x21500063","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T20:55:11Z","timestamp":1609188911000},"page":"113-129","source":"Crossref","is-referenced-by-count":38,"title":["Epigallocatechin Gallate Reduces Homocysteine-Caused Oxidative Damages through Modulation SIRT1\/AMPK Pathway in Endothelial Cells"],"prefix":"10.1142","volume":"49","author":[{"given":"Pei-Ying","family":"Pai","sequence":"first","affiliation":[{"name":"School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan, ROC"},{"name":"Division of Cardiology, China Medical University Hospital, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Ching","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Hung","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Yih","family":"Wu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University, Taichung, Taiwan, ROC"},{"name":"Department of Physical and Rehabilitation Medicine, Asia University Hospital, Taichung, Taiwan, ROC"},{"name":"Department of Physical Therapy, College of Medical and Health Science, Asia University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu-I","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, Hungkuang University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Post-Baccalaureate Veterinary Medicine, College of Medical and Health Science, Asia University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Ling","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, China Medical University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Ming","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, China Medical University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-An","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Health and Leisure Management, Yuanpei University of Medical Technology, Hsinchu, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu-Chung","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, College of Medical and Health Science, Asia University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-Ling","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC"},{"name":"Institute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"219","published-online":{"date-parts":[[2020,12,26]]},"reference":[{"key":"S0192415X21500063BIB001","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1016\/j.biopha.2018.11.086","volume":"109","author":"Al Hroob A.M.","year":"2019","journal-title":"Biomed. 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