{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T09:17:34Z","timestamp":1793524654433,"version":"4.5.0"},"reference-count":38,"publisher":"World Scientific Pub Co Pte Ltd","issue":"08","funder":[{"name":"Natural Science Foundation of Guangxi","award":["2018GXNSFAA050110"],"award-info":[{"award-number":["2018GXNSFAA050110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Chin. Med."],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Myocardial ischemia\/reperfusion injury (MIRI) is the major cause of myocardial cell damage in acute myocardial infarction, and its treatment remains a clinical challenge. Ginsenoside Rb1 showed protective effects on the cardiovascular system; however, the underlying mechanism remains largely unclear. Effects of Ginsenoside Rb1 on rat MIRI-induced myocardial infarct size were evaluated through TTC staining. TUNEL assay and flow cytometry analysis were employed to estimate cell apoptosis. Apoptosis, autophagy and PI3K\/Akt\/mTOR pathway-related proteins were estimated via western blot. Expression of Beclin1 in myocardial tissues were examined by immunohistochemical analysis. Expression levels of IL-1[Formula: see text], TNF-[Formula: see text] and IL-6 were tested by enzyme-linked immunosorbent assay (ELISA). Here, we found that Ginsenoside Rb1 treatment not only alleviated MIRI in rats but also protected H9C2 cells against hypoxia\/reoxygenation induced damage. Ginsenoside Rb1 abolished the MIRI-induced activation of autophagy. Meanwhile, we found that treatment of 3-MA (autophagy inhibitor) could enhance the protective effects of Ginsenoside Rb1 on H9C2 cells during H\/R. Moreover, Ginsenoside Rb1 treatment resulted in the activation of the PI3K\/Akt\/mTOR pathway, and treatment of LY294002 (PI3K\/Akt pathway repressor) abolished the protective effects of Ginsenoside Rb1 on myocardial in vitro and in vivo. Our results suggest that Ginsenoside Rb1 functions as a protector against MIRI by repressing cardiomyocyte autophagy through the PI3K\/Akt\/mTOR signaling pathway.<\/jats:p>","DOI":"10.1142\/s0192415x21500907","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T23:44:45Z","timestamp":1636933485000},"page":"1913-1927","source":"Crossref","is-referenced-by-count":158,"title":["Ginsenoside Rb1 Inhibits Cardiomyocyte Autophagy via PI3K\/Akt\/mTOR Signaling Pathway and Reduces Myocardial Ischemia\/Reperfusion Injury"],"prefix":"10.1142","volume":"49","author":[{"given":"Guo-Wei","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Science and Technology, Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, P. R. 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