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Chin. Med."],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>ECa 233 is a standardized extract of Centella asiatica (CA), an herb widely used in traditional Chinese and Ayurvedic medicine. Previous studies reported that ECa 233 enhanced memory retention and synaptic plasticity in the hippocampus of healthy rats. Because of this, we became curious whether ECa 233 has a therapeutic effect on the fear memory deficit in the triple transgenic Alzheimer\u2019s disease (3xTg-AD) model mice. Fear memory is a crucial emotional memory for survival that is found to be impaired in patients with early-onset Alzheimer\u2019s disease (AD). In this study, we orally administered ECa 233 (doses: 10, 30, and 100[Formula: see text]mg\/kg) to 3xTg-AD mice, who were five months old, for 30 consecutive days. We found that ECa 233 prevented a cued fear memory deficit and enhanced hippocampal long-term potentiation (LTP) in 3xTg-AD mice. Subsequent proteomic and western blot analyses revealed increased expression levels of the molecules related to LTP induction and maintenance, including brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and its network proteins, and extracellular signal-regulated kinase 1 and 2 (ERK1 and 2) in the hippocampi and amygdala of 3xTg-AD mice after ECa 233 pre-treatment. Our results indicate that ECa 233 is a promising potential herbal standardized extract that could be used in preventing the fear memory deficit and synaptic dysfunction before the early onset of AD.<\/jats:p>","DOI":"10.1142\/s0192415x23500179","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T16:03:08Z","timestamp":1674662588000},"page":"329-353","source":"Crossref","is-referenced-by-count":7,"title":["Standardized Extract of<i>Centella asiatica<\/i>Prevents Fear Memory Deficit in 3xTg-AD Mice"],"prefix":"10.1142","volume":"51","author":[{"given":"Tanita","family":"Pairojana","sequence":"first","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarayut","family":"Phasuk","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuree H.","family":"Tantisira","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Burapha University, 169 Long Hard-Bangsaen Rd., Saensuk, Amphoe Muang, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Chi","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narawut","family":"Pakaprot","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkoknoi, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supin","family":"Chompoopong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkoknoi, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutisa","family":"Nudmamud-Thanoi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, 99-9, Tha-pho, Muang-Phitsanulok, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Ming","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"219","published-online":{"date-parts":[[2023,1,25]]},"reference":[{"key":"S0192415X23500179BIB001","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.neuron.2005.01.040","volume":"45","author":"Billings L. 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