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Image TS represents the evolution of anatomy over time, but different anatomies may have different structural characteristics and temporal paths. Therefore, separating the time-dependent path difference and time-independent basic anatomy\/shape changes is important when comparing two image TS to understand the causes of the observed differences better. A method to untangle and quantify the path and shape difference between the TS is presented in this paper. The proposed method is evaluated with simulated and adult and fetal neuro templates. 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