{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:30:15Z","timestamp":1767839415979,"version":"3.49.0"},"reference-count":63,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:p> Peptide toxins have been valuable probes in efforts to identify amino acid residues that line the permeation pathway of cation-selective channels. However, no peptide toxins have been identified that interact with known anion-selective channels such as the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR channels are expressed in epithelial cells and are associated with several genetic disorders, including cystic fibrosis and polycystic kidney disease. Several organic inhibitors have been used to investigate the structure of the Cl<jats:sup>\u2212<\/jats:sup> permeation pathway in CFTR. However, investigations of the wider cytoplasmic vestibule have been hindered by the lack of a high-affinity blocker that interacts with residues in this area. In this study we show that venom of the scorpion Leiurus quinquestriatus hebraeus reversibly inhibits CFTR, in a voltage-independent manner, by decreasing single-channel mean burst duration and open probability only when applied to the cytoplasmic surface of phosphorylated channels. Venom was able to decrease burst duration and open probability even when CFTR channels were locked open by treatment with either vanadate or adenosine 5\u2032-(\u03b2,\u03b3-imido)triphosphate, and block was strengthened on reduction of extracellular Cl<jats:sup>\u2212<\/jats:sup> concentration, suggesting inhibition by a pore-block mechanism. Venom had no effect on ATP-dependent macroscopic opening rate in channels studied by inside-out macropatches. Interestingly, the inhibitory activity was abolished by proteinase treatment. We conclude that a peptide toxin contained in the scorpion venom inhibits CFTR channels by a pore-block mechanism; these experiments provide the first step toward isolation of the active component, which would be highly valuable as a probe for CFTR structure and function. <\/jats:p>","DOI":"10.1152\/ajpcell.00162.2004","type":"journal-article","created":{"date-parts":[[2004,7,8]],"date-time":"2004-07-08T00:23:57Z","timestamp":1089246237000},"page":"C1328-C1341","source":"Crossref","is-referenced-by-count":20,"title":["Inhibition of CFTR channels by a peptide toxin of scorpion venom"],"prefix":"10.1152","volume":"287","author":[{"given":"Matthew D.","family":"Fuller","sequence":"first","affiliation":[]},{"given":"Zhi-Ren","family":"Zhang","sequence":"additional","affiliation":[]},{"given":"Guiying","family":"Cui","sequence":"additional","affiliation":[]},{"given":"Julia","family":"Kubanek","sequence":"additional","affiliation":[]},{"given":"Nael A.","family":"McCarty","sequence":"additional","affiliation":[]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Aiyer J, Rizzi JP, Gutman GA, and Chandy KG. 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