{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:57:33Z","timestamp":1780451853101,"version":"3.54.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p> TALK-1a, originally isolated from human pancreas, is a member of the tandem-pore K<jats:sup>+<\/jats:sup> channel family. We identified and characterized three novel splice variants of TALK-1 from human pancreas. The cDNAs of TALK-1b, TALK-1c, and TALK-1d encode putative proteins of 294, 322, and 262 amino acids, respectively. TALK-1a and TALK-1b possessed all four transmembrane segments, whereas TALK-1c and TALK-1d lacked the fourth transmembrane domain because of deletion of exon 5. Northern blot analysis showed that among the 15 tissues examined, TALK-1 was expressed mainly in the pancreas. TALK-1a and TALK-1b, but not TALK-1c and TALK-1d, could be functionally expressed in COS-7 cells. Like TALK-1a, TALK-1b was a K<jats:sup>+<\/jats:sup>-selective channel that was active at rest. Single-channel openings of TALK-1a and TALK-1b were extremely brief such that the mean open time was &lt;0.2 ms. In symmetrical 150 mM KCl, the apparent single-channel conductances of TALK-1a and TALK-1b were 23 \u00b1 3 and 21 \u00b1 2 pS at \u201360 mV and 11 \u00b1 2 and 10 \u00b1 2 pS at +60 mV, respectively. TALK-1b whole cell current was inhibited 31% by 1 mM Ba<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> and 71% by 1 mM quinidine but was not affected by 1 mM tetraethylammonium, 1 mM Cs<jats:sup>+<\/jats:sup>, and 100 \u03bcM 4-aminopyridine. Similar to TALK-1a, TALK-1b was sensitive to changes in external pH. Acid conditions inhibited and alkaline conditions activated TALK-1a and TALK-1b, with a K<jats:sub>1\/2<\/jats:sub> at pH 7.16 and 7.21, respectively. These results indicate that at least two functional TALK-1 variants are present and may serve as background K<jats:sup>+<\/jats:sup> currents in certain cells of the human pancreas. <\/jats:p>","DOI":"10.1152\/ajpcell.00601.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:16:02Z","timestamp":1368490562000},"page":"C529-C538","source":"Crossref","is-referenced-by-count":41,"title":["Functional properties of four splice variants of a human pancreatic  tandem-pore K<sup>+<\/sup> channel, TALK-1"],"prefix":"10.1152","volume":"285","author":[{"given":"Jaehee","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawon","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Ashmole I,  Goodwin PA, and Stanfield PR. 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Mol Endocrinol 16: 621\u2013629,  2002.","DOI":"10.1210\/me.16.3.621"},{"key":"REF8","doi-asserted-by":"crossref","unstructured":"Decher N, Maier  M, Dittrich W, Gassenhuber J, Bruggemann A, Busch AE, and Steinmeyer K.  Characterization of TASK-4, a novel member of the pH-sensitive, two-pore  domain potassium channel family. FEBS Lett 492: 84\u201389,  2001.","DOI":"10.1016\/S0014-5793(01)02222-0"},{"key":"REF9","doi-asserted-by":"crossref","unstructured":"Duprat M,  Lesage F, Fink M, Reyes R, Heurteaux C, and Lazdunski M. TASK, a human  background K+ channel to sense external pH variations near  physiological pH. EMBO J 16:  5464\u20135471, 1997.","DOI":"10.1093\/emboj\/16.17.5464"},{"key":"REF10","doi-asserted-by":"crossref","unstructured":"Fink M, Duprat  F, Lesage F, Reyes R, Romey G, Heurteaux C, and Lazdunski M. Cloning,  functional expression and brain localization of a novel unconventional outward  rectifier K+ channel. EMBO J 15: 6854\u20136862,  1996.","DOI":"10.1002\/j.1460-2075.1996.tb01077.x"},{"key":"REF11","doi-asserted-by":"crossref","unstructured":"Fink M, Lesage  F, Duprat F, Heurteaux C, Reyes R, Fosset M, and Lazdunski M. A neuronal  two P domain K+ channel stimulated by arachidonic acid and  polyunsaturated fatty acids. EMBO J 17: 3297\u20133308,  1998.","DOI":"10.1093\/emboj\/17.12.3297"},{"key":"REF12","doi-asserted-by":"crossref","unstructured":"Girard C,  Duprat F, Terrenoire C, Tinel N, Fosset M, Romey G, Lazdunski M, and Lesage  F. Genomic and functional characteristics of novel human pancreatic 2P  domain K+ channels. Biochem Biophys Res  Commun 282:  249\u2013256, 2001.","DOI":"10.1006\/bbrc.2001.4562"},{"key":"REF13","doi-asserted-by":"crossref","unstructured":"Goldstein SAN,  Bockenhauer D, and Zilberberg N. Potassium leak channels and the KCNK  family of two-P-domain subunits. Nat Neurosci 2: 175\u2013184,  2001.","DOI":"10.1038\/35058574"},{"key":"REF14","doi-asserted-by":"crossref","unstructured":"Gu W,  Schlichth\u00f6rl G, Hirsch JR, Engels H, Karschin C, Karschin A, Derst C,  Steinlein OK, and Daut J. Expression pattern and functional  characteristics of two novel splice variants of the two-pore-domain potassium  channel TREK-2. J Physiol 539:  657\u2013668, 2002.","DOI":"10.1113\/jphysiol.2001.013432"},{"key":"REF15","doi-asserted-by":"crossref","unstructured":"Han J, Truell  J, Gnatenco C, and Kim D. Characterization of four types of background  potassium channels in rat cerebellar granule neurons. J  Physiol 542:  431\u2013444, 2002.","DOI":"10.1113\/jphysiol.2002.017590"},{"key":"REF16","doi-asserted-by":"crossref","unstructured":"Honore E,  Maingret F, Lazdunski M, and Patel AJ. An intracellular proton sensor  commands lipid- and mechano-gating of the K+ channel TREK-1.  EMBO J 21:  2968\u20132976, 2002.","DOI":"10.1093\/emboj\/cdf288"},{"key":"REF17","doi-asserted-by":"crossref","unstructured":"Kim D and  Clapham DE. Potassium channels in cardiac cells activated by arachidonic  acid and phospholipids. Science 244: 1174\u20131176,  1989.","DOI":"10.1126\/science.2727703"},{"key":"REF18","doi-asserted-by":"crossref","unstructured":"Kim D and  Gnatenco C. TASK-5, a new member of the tandem-pore K+ channel  family. Biochem Biophys Res Commun 284: 923\u2013930,  2001.","DOI":"10.1006\/bbrc.2001.5064"},{"key":"REF19","doi-asserted-by":"crossref","unstructured":"Kim D, Sladek  CD, Aquado-Velasco C, and Mathiasen JR. Arachidonic acid activation of a  new family of K+ channels in cultured rat neuronal cells.  J Physiol 484.3:  643\u2013660, 1995.","DOI":"10.1113\/jphysiol.1995.sp020693"},{"key":"REF20","doi-asserted-by":"crossref","unstructured":"Kim Y, Bang H,  Gnatenco C, and Kim D. Synergistic interaction and the role of C-terminus  in the activation of TRAAK K+ channels by pressure, free fatty  acids and alkali. Pfl\u00fcgers Arch 442: 64\u201372,  2001.","DOI":"10.1007\/s004240000496"},{"key":"REF21","doi-asserted-by":"crossref","unstructured":"Kim Y, Bang H,  and Kim D. Task-3, a new member of the tandem pore K+ channel  family. J Biol Chem 275:  9340\u20139347, 2000.","DOI":"10.1074\/jbc.275.13.9340"},{"key":"REF22","doi-asserted-by":"crossref","unstructured":"Kim Y, Bang HW,  and Kim D. TBAK-1 and TASK-1, two-pore K+ channel subunits:  kinetic properties and expression in rat heart. Am J Physiol Heart  Circ Physiol 277:  H1669\u2013H1678, 1999.","DOI":"10.1152\/ajpheart.1999.277.5.H1669"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Kim Y, Gnatenco  C, Bang H, and Kim D. Localization of TREK-2 K+ channel domains  that regulate channel kinetics and sensitivity to pressure, fatty acids and  pHi. Pfl\u00fcgers Arch 442: 952\u2013960,  2001.","DOI":"10.1007\/s004240100626"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Lesage F,  Guillemare E, Fink M, Duprat F, Lazdunski M, Romey G, and Barhanin J.  TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with  a novel structure. EMBO J 15:  1004\u20131011, 1996.","DOI":"10.1002\/j.1460-2075.1996.tb00437.x"},{"key":"REF25","doi-asserted-by":"crossref","unstructured":"Lesage F and  Lazdunski M. Molecular and functional properties of two-pore-domain  potassium channels. Am J Physiol Renal Physiol 279: F793\u2013F801,  2000.","DOI":"10.1152\/ajprenal.2000.279.5.F793"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Maingret F,  Fosset M, Lesage F, Lazdunski M, and Honore E. TRAAK is a mammalian  neuronal mechano-gated K+ channel. J Biol  Chem 274:  1381\u20131387, 1999.","DOI":"10.1074\/jbc.274.3.1381"},{"key":"REF27","doi-asserted-by":"crossref","unstructured":"Nichols CG and  Koster JC. Diabetes and insulin secretion: whither KATP?  Am J Physiol Endocrinol Metab 283: E403\u2013E412,  2002.","DOI":"10.1152\/ajpendo.00168.2002"},{"key":"REF28","doi-asserted-by":"crossref","unstructured":"Patel AJ and  Honore E. Properties and modulation of mammalian 2P domain K+  channels. Trends Neurosci 24:  339\u2013346, 2001.","DOI":"10.1016\/S0166-2236(00)01810-5"},{"key":"REF29","doi-asserted-by":"crossref","unstructured":"Patel AJ,  Honore E, Maingret F, Lesage F, Fink M, Duprat F, and Lazdunski M. A  mammalian two pore domain mechano-gated S-like K+ channel.  EMBO J 17:  4283\u20134290, 1998.","DOI":"10.1093\/emboj\/17.15.4283"},{"key":"REF30","doi-asserted-by":"crossref","unstructured":"Patel AJ,  Lazdunski M, and Honore E. Lipid and mechano-gated 2P domain K+  channels. Curr Opin Cell Biol 13: 422\u2013428,  2001.","DOI":"10.1016\/S0955-0674(00)00231-3"},{"key":"REF31","doi-asserted-by":"crossref","unstructured":"Patel AJ,  Maingret F, Magnone V, Fosset M, Lazdunski M, and Honore E. TWIK-2, an  inactivating 2P domain K+ channel. J Biol  Chem 275:  28722\u201328730, 2000.","DOI":"10.1074\/jbc.M003755200"},{"key":"REF32","doi-asserted-by":"crossref","unstructured":"Pountney DJ,  Gulkarov I, Vega-Saenz de Miera E, Holmes D, Saganich M, Rudy B, Artman M, and  Coetzee WA. Identification and cloning of TWIK-originated similarity  sequence (TOSS): a novel human 2-pore K+ channel principal subunit.  FEBS Lett 450:  191\u2013196, 1999.","DOI":"10.1016\/S0014-5793(99)00495-0"},{"key":"REF33","doi-asserted-by":"crossref","unstructured":"Rajan S,  Wischmeyer E, Karschin C, Preisig-Muller R, Grzeschik KH, Daut J, Karschin A,  and Derst C. THIK-1 and THIK-2, a novel subfamily of tandem pore domain  K+ channels. J Biol Chem 276: 7302\u20137311,  2001.","DOI":"10.1074\/jbc.M008985200"},{"key":"REF34","doi-asserted-by":"crossref","unstructured":"Salinas M,  Reyes R, Lesage F, Fosset M, Heurteaux C, Romey G, and Lazdunski M.  Cloning of a new mouse two-P domain channel subunit and a human homologue with  a unique pore structure. J Biol Chem 274: 11751\u201311760,  1999.","DOI":"10.1074\/jbc.274.17.11751"},{"key":"REF35","doi-asserted-by":"crossref","unstructured":"Sharma N, Crane  A, Gonzalez G, Bryan J, and Aguilar-Bryan L. Familial hyperinsulinism and  pancreatic beta-cell ATP-sensitive potassium channels. Kidney  Int 57:  803\u2013808, 2000.","DOI":"10.1046\/j.1523-1755.2000.00918.x"},{"key":"REF36","doi-asserted-by":"crossref","unstructured":"Talley EM and  Bayliss DA. Modulation of TASK-1 (Kcnk3) and TASK-3 (Kcnk9) potassium  channels: volatile anesthetics and neurotransmitters share a molecular site of  action. J Biol Chem 277:  17733\u201317742, 2002.","DOI":"10.1074\/jbc.M200502200"},{"key":"REF37","doi-asserted-by":"crossref","unstructured":"Washburn CP,  Sirois JE, Talley EM, Guyenet PG, and Bayliss DA. Serotonergic raphe  neurons express TASK channel transcripts and a TASK-like pH- and  halothane-sensitive K+ conductance. J  Neurosci 22:  1256\u20131265, 2002.","DOI":"10.1523\/JNEUROSCI.22-04-01256.2002"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00601.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:04:10Z","timestamp":1718651050000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00601.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1152\/ajpcell.00601.2002"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00601.2002","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]}}}