{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:19:51Z","timestamp":1758403191000},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1978,5,1]]},"abstract":"<jats:p> The possible role of microtubules in milk production was examined in mammary gland slices from lactating guinea pigs. Colchicine, 10(-5)-10(-4) M, depressed lactose secretion within 15 min, maintaining maximal inhibition over 2.5 h, accompanied by retention of lactose within the slices. In colchicine dose-response studies (2 h, 10(-8)-10(-5) M), secretion was depressed 22% by 10(-5) M, whereas tissue lactose increased with dose up to +25% at 10(-5) M. Lactose synthesis was inhibited 3-19% without correlation to colchicine concentration. In another study, incubation with 10(-5) M lumicolchicine yielded one-third less inhibition of secretion than 10(-5) M colchicine with no increase in tissue lactose. Both drugs depressed synthesis 31%. Lactose secretion showed a negative correlation with 10(-7)-10(-4) M vincristine yielding a maximal 66% inhibition at 10(-4) M, whereas tissue retention showed a linear increase with concentration up to 151% of control at 10(-4) M. Effects on synthesis were sporadic. These data suggest that microtubules have a role in facilitating the transport and\/or secretion of lactose and perhaps other milk components. <\/jats:p>","DOI":"10.1152\/ajpcell.1978.234.5.c177","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T04:41:04Z","timestamp":1514090464000},"page":"C177-C180","source":"Crossref","is-referenced-by-count":16,"title":["Inhibition of mammary gland lactose secretion by colchicine and vincristine"],"prefix":"10.1152","volume":"234","author":[{"given":"M. A.","family":"Guerin","sequence":"first","affiliation":[]},{"given":"R. F.","family":"Loizzi","sequence":"additional","affiliation":[]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1978.234.5.C177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:28:32Z","timestamp":1718641712000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1978.234.5.C177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1978,5,1]]}},"alternative-id":["10.1152\/ajpcell.1978.234.5.C177"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1978.234.5.c177","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,5,1]]}}}