{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T23:28:45Z","timestamp":1747783725883},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,11,1]]},"abstract":"<jats:p> We tested whether recognition of bone-related peptides regulates intracellular Ca2+ concentration ([Ca2+]i) of giant cell tumor of bone (GCT). [Ca2+]i was measured in single cells by fura 2 fluorometry. GCT cells were sensitive to bone sialoprotein-II (BSP-II), osteopontin (OPN), and related fragments. Responses consisted of a prompt increase of [Ca2+]i, mostly transient, with a peak followed by a rapid return toward baseline. Responses were not mimicked by bovine plasma fibronectin. Sensitivity of GCT cells to bone peptides was specific, since BALB\/3T3 fibroblasts and U-937 histiocytic lymphoma cells with monocytic phenotype failed to respond to BSP-II and OPN fragments. GRGDSP synthetic esapeptide, carrying the Arg-Gly-Asp adhesive motif, and GRGESP (Asp replaced by Glu), but not the GRADSP (Gly replaced by Ala), were active in inducing [Ca2+]i transients as well. Responses were observed also in cells treated with the BSP-II 1C fragment, lacking any known adhesive sequence, indicating that the active peptides inducing [Ca2+]i increments may be multiple. Sensitivity to extracellular matrix peptides was present in a variable fraction of the cells and was downregulated on long-term culture. The mechanism inducing [Ca2+]i elevations was mostly related to Ca2+ release from thapsigargin-sensitive intracellular pools. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.5.c1289","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:47:42Z","timestamp":1514126862000},"page":"C1289-C1297","source":"Crossref","is-referenced-by-count":40,"title":["Immediate cell signal by bone-related peptides in human osteoclast-like cells"],"prefix":"10.1152","volume":"265","author":[{"given":"R.","family":"Paniccia","sequence":"first","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Colucci","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Grano","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Serra","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Z.","family":"Zallone","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Teti","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, School of Pharmacy, University of Bari,Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.5.C1289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:26Z","timestamp":1718653346000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.5.C1289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,11,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.5.C1289"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.5.c1289","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,11,1]]}}}