{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:28:06Z","timestamp":1761060486216},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,11,1]]},"abstract":"<jats:p> Expression of Na(+)-glucose cotransporters of the SGLT-1 type by Xenopus laevis oocytes increased the osmotic water permeability (Pf) of oocytes by a factor of 1.9-2.8, in the presence and in the absence of 5 mM extracellular glucose. The Pf increase was correlated with the amount of SGLT-1 cRNA injected. In oocytes expressing SGLT-1, either addition of phlorizin to the medium or the replacement of Na+ by choline inhibited the uptake of methyl-alpha-D-glucopyranoside, a specific substrate for SGLT-1, and returned oocyte Pf to its level in uninjected oocytes. Phlorizin inhibited the SGLT-1-attributable increase in Pf with an inhibition constant (Ki) of 6.1 microM, a value analogous to the Ki for phlorizin inhibition of sugar uptake. However, neither the presence of phlorizin nor the absence of extracellular Na+ significantly affected the increase in Pf elicited in oocytes expressing GLUT-1, a facilitative glucose transporter. These findings suggest that SGLT-1 forms a pore that allows the transmembrane passage of water and that water and glucose traverse the protein through this pore. The finding that removal of extracellular Na+ abolishes the increase in Pf attributable to SGLT-1 suggests that extracellular Na+ is required to maintain patency of this transporter's water-permeable transmembrane pore. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.5.c1774","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:59:53Z","timestamp":1514145593000},"page":"C1774-C1779","source":"Crossref","is-referenced-by-count":39,"title":["Sodium-glucose cotransporters display sodium- and phlorizin-dependent water permeability"],"prefix":"10.1152","volume":"271","author":[{"given":"J. D.","family":"Loike","sequence":"first","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"S.","family":"Hickman","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"K.","family":"Kuang","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"M.","family":"Xu","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"L.","family":"Cao","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"J. C.","family":"Vera","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"S. C.","family":"Silverstein","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]},{"given":"J.","family":"Fischbarg","sequence":"additional","affiliation":[{"name":"Rover Laboratory, Department of Physiology and Cellular Biophysics,Columbia University, New York 10032, USA."}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.5.C1774","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:36:46Z","timestamp":1718653006000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.5.C1774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,11,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.5.C1774"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.5.c1774","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"type":"print","value":"0363-6143"},{"type":"electronic","value":"1522-1563"}],"subject":[],"published":{"date-parts":[[1996,11,1]]}}}